Part II. Studies on fractionated purified materials using ion exchange resins (Studies on Anti-tuberculous Low Molecular Factors in Various Organs of Rabbits)

Abstract

1) Extracts from the muscle, the liver, the kidney and the lung of rabbits were fractionated by using systematically the ion exchange resins (Amberlite IR-120 and IRA-400) into four fractions, that is, Fraction I; amino acid or peptide, Fraction II; organic base, Fraction III; organic acid, Fraction IV; sugar. 2) The fractions which have tuberculostatic activity, more or less, were as follows; Fraction I in these four organs, Fraction III in muscle and lung, Fraction IV in liver. 3) The active fraction (muscle, liver) acted more inhibitingly on the growth of bovine type tubercle bacilli and less on the avian type, than on the human type. 4) The tuberculo-inhibitory action of the active factors was thought to be bactericidal rather than bacteriostatic. 5) The tuberculostatic activity of each active fraction was not increased by immunization. 6) Chemical examinations of each fraction were carried out by using chromatography, but identification of each component could not be finished.