<Original>Cell-Free Extraction of Oxaloacetase from White-Rot Fungi, Including Coriolus versicolor

Abstract

Oxaloacetase, which catalyzes production of oxalic acid from oxaloacetic acid, was cell-free extracted for the first time from a white-rot fungus, Coriolus versicolor. Several other wood destroying fungi were grown on glucose and their oxaloacetase activities in cell-free extracts were compared. It was shown that the amounts of oxalic acid accumulated in the culture media reached the maximal value on day 7 of the cultivation, and decreased thereafter. The enzyme activity gradually increased until day 7 and more rapidly increased thereafter until day 10.