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Title: Generation of knock-in mice carrying third cones with spectral sensitivity different from S and L cones
Authors: Onishi, Akishi
Hasegawa, Jun
Imai, Hiroo  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0003-0729-0322 (unconfirmed)
Chisaka, Osamu  kyouindb  KAKEN_id
Ueda, Yoshiki
Honda, Yoshihito
Tachibana, Masao
Shichida, Yoshinori  kyouindb  KAKEN_id
Author's alias: 七田, 芳則
Keywords: spectral tuning
X-inactivation
cone pigments
knock-in mice
color vision
Issue Date: Oct-2005
Publisher: Zoological Society of Japan
Journal title: Zoological Science
Volume: 22
Issue: 10
Start page: 1145
End page: 1156
Abstract: Red-green color vision in primates is unique in the sense that it is mediated by two photoreceptor cells that are indistinguishable in all aspects except for their visual pigments. In order to generate an animal model for investigation of the interaction between red-green inputs at the molecular level, we applied knock-in technology and X-chromosome inactivation machinery to make a mouse model with cone cells possessing visual pigments with different spectral sensitivities. We introduced a S308A point mutation into the Green opsin gene allele on the X-chromosome. This manipulation generated a 24 nm red-shift of absorption maximum in the cone pigment with negligible functional differences in other molecular properties. Amplitudes of responses in ERG and ganglion cell recordings of homozygotes were similar to those of wild-types, although the spectral sensitivities differed. Heterozygotes showed variable spectral sensitivities of ganglion cell responses due to the different integration of the native and the S308A cone inputs on the dendritic fields. In situ hybridization experiments showed that cone cells with respective pigments formed patch-like clusters of specific L cone-types, approximately 30 mu m in diameter, which were randomly distributed in the dorsal region of the retinas. Since the patch-like clustering was arranged by X-inactivation, such clustering could be present in the peripheral retinas of New World monkeys with polymorphic L pigments, indicating that our mice would be a suitable model to study evolution of the mammalian color vision system.
Rights: (c) 日本動物学会 / Zoological Society of Japan
URI: http://hdl.handle.net/2433/57194
DOI(Published Version): 10.2108/zsj.22.1145
Appears in Collections:Zoological Science

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