ダウンロード数: 525
タイトル: | Construction of a cDNA microarray derived from the ascidian Ciona intestinalis |
著者: | Azumi, Kaoru Takahashi, Hiroki Miki, Yasufumi Fujie, Manabu Usami, Takeshi Ishikawa, Hisayoshi Kitayama, Atsusi Satou, Yutaka https://orcid.org/0000-0001-5193-0708 (unconfirmed) Ueno, Naoto Satoh, Nori |
著者名の別形: | 佐藤, 矩行 佐藤, ゆたか |
キーワード: | ascidian Ciona intestinalis cDNA microarray gene expression development |
発行日: | Oct-2003 |
出版者: | Zoological Society of Japan |
誌名: | Zoological Science |
巻: | 20 |
号: | 10 |
開始ページ: | 1223 |
終了ページ: | 1229 |
抄録: | A cDNA microarray was constructed from a basal chordate, the ascidian Ciona intestinalis. The draft genome of Ciona has been read and inferred to contain similar to16,000 protein-coding genes, and cDNAs for transcripts of 13,464 genes have been characterized and compiled as the "Ciona intestinalis Gene Collection Release I". In the present study, we constructed a cDNA microarray of these 13,464 Ciona genes. A preliminary experiment with Cy3- and Cy5-labeled probes showed extensive differential gene expression between fertilized eggs and larvae. In addition, there was a good correlation between results obtained by the present microarray analysis and those from previous EST analyses. This first microarray of a large collection of Ciona intestinalis cDNA clones should facilitate the analysis of global gene expression and gene networks during the embryogenesis of basal chordates. |
著作権等: | (c) 日本動物学会 / Zoological Society of Japan |
URI: | http://hdl.handle.net/2433/57202 |
DOI(出版社版): | 10.2108/zsj.20.1223 |
出現コレクション: | Zoological Science |
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