ダウンロード数: 451
タイトル: | Estrogen inhibits development of yolk veins and causes blood clotting in transgenic medaka fish overexpressing estrogen receptor |
著者: | Kawamura, Toshiyuki Sakai, Shuichi Omura, Seiichi Hori-e, Ryota Kawahara, Tetsuya Kinoshita, Masato https://orcid.org/0000-0003-3936-7371 (unconfirmed) Yamashita, Ichiro |
著者名の別形: | 木下, 政人 |
キーワード: | transgenic medaka fish blood clotting yolk vein estrogen estrogen receptor |
発行日: | Dec-2002 |
出版者: | Zoological Society of Japan |
誌名: | Zoological Science |
巻: | 19 |
号: | 12 |
開始ページ: | 1355 |
終了ページ: | 1361 |
抄録: | We established three transgenic medaka fish lines overexpressing the medaka estrogen receptor under the constitutive medaka beta-actin promoter. The transgenic embryos became hypersensitive to estrogens (17beta-estradiol and 17alpha-ethinylestradiol), and failed to develop yolk veins while blood clots formed in the blood island within 3 days after exposure to the estrogens. The embryos developed normally if exposed to estrogen after an early neurula stage, suggesting that the sensitive stage is before neurulation. The developmental defects were recovered by incubation with an anti-estrogen, tamoxifen. These results indicate that activation of estrogen receptor caused the estrogen-induced, developmental defects. Our results show that the transgenic embryos can be used to assay the blood clotting activity of estrogenic compounds in vivo. |
著作権等: | (c) 日本動物学会 / Zoological Society of Japan |
URI: | http://hdl.handle.net/2433/57210 |
DOI(出版社版): | 10.2108/zsj.19.1355 |
出現コレクション: | Zoological Science |
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