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Title: Surface coat proteins of the pine wood nematode, Bursaphelenchus xylophilus: Profiles of stage- and isolate-specific characters
Authors: Shinya, Ryoji
Takeuchi, Yuko  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0002-4503-033X (unconfirmed)
Miura, Natsuko
Kuroda, Kouichi  kyouindb  KAKEN_id
Ueda, Mitsuyoshi  kyouindb  KAKEN_id
Futai, Kazuyoshi
Author's alias: 新屋, 良治
Keywords: Glycan
Glycoprotein
Lectin blot
SDS-PAGE
WGA
Issue Date: 2009
Publisher: Brill Academic Publishers
Journal title: Nematology
Volume: 11
Issue: 3
Start page: 429
End page: 438
Abstract: The present study was made to determine the binding patterns of several lectins to the surface coat (SC) proteins of various isolates and developmental stages of the pine wood nematode (PWN), Bursaphelenchus xylophilus. Also, the detailed characteristics of the SC proteins were profiled by using molecular techniques. The lectin-binding study demonstrated the stage-specific characters of SC in binding to the lectin, wheat germ agglutinin (WGA). WGA binding was observed only to the outer surfaces of third-stage propagative juveniles and to the egg shells, and this occurred more frequently in virulent than in avirulent PWN isolates. A greater variety of lectins bound to eggs than to any other life stage. For characterisation, the SC proteins extracted were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and analysed by lectin blotting. The results showed that the carbohydrate and protein patterns of the SC of the PWN changed during nematode development.
Rights: c Koninklijke Brill NV, Leiden, 2009.
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
URI: http://hdl.handle.net/2433/89650
DOI(Published Version): 10.1163/156854109X447006
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