Angiogenin expression in superficial bladder cancer

Abstract

20名の表在性膀胱腫瘍患者の腫瘍組織内と正常組織内各々のangiogenin濃度をサンドウィッチELISA法を用い測定し, 膀胱組織内におけるangiogeninの局在を明らかにする目的で免疫染色を行った.腫瘍組織内のangiogenin濃度は正常組織内の濃度と比較して有意に低下し, angiogeninの発現は正常移行上皮に高く, 腫瘍組織内で明らかに低下していた.膀胱腫瘍患者の組織内でのangiogeninの発現は正常組織と比較すると腫瘍組織内で有意に低下していた.この結果は, 膀胱腫瘍の発育の過程ではangiogeninは他の血管新生因子とは異なる役割を担っている可能性が示唆された

The concentration of angiogenin in the tumor tissues and corresponding normal tissues of 20 superficial bladder cancer patients was measured using a sandwich enzyme immunoassay (ELISA). In addition, immunohistochemical assays were performed in order to clarify the localization of angiogenin expression in bladder tissue. The mean concentration of angiogenin in the carcinoma tissues was significantly lower than that in the corresponding normal tissues (P < 0.001). Angiogenin expression was weak in the bladder cancer cells. The present results show that the expression of angiogenin is lower in superficial bladder cancer tissues than in corresponding normal tissues. The biological role of angiogenin in carcinogenesis of bladder cancer may be different from those of other angiogenic factors.