Interleukin-1 alpha and interleukin-1 beta production in peripheral whole blood from patients with urological cancer

Abstract

対象は60例の泌尿器科癌患者.その内訳は膀胱癌30例, 腎癌12例, 前立腺癌18例であった.また, 対照群は健常者16例.測定方法はヘパリン採血した全血0.1mlずつを, 6段階(0, 0.1, 0.3, 1, 3, 5μg/ml)の濃度のリポポリサッカライド添加無血清培地にて24時間培養し, その上清をIL-1α, IL-1βに対するモノクローナル抗体を用いたELISA法にて測定した.リポポリサッカライドを含まない条件での全血1mlあたりのIL-1α, IL-1β産生量は, 膀胱癌患者, 腎癌患者, 前立腺癌患者では健常者と比較して有意差を認めなかった.次に5段階濃度のリポポリサッカライドを用いた場合, 膀胱癌患者, 腎癌患者, 前立腺癌患者, 健常者におけるIL-1α, IL-1β産生量はdose responseを示した.また, IL-1βの産生量はIL-1αより高値であった.しかし, 膀胱癌患者, 腎癌患者, 前立腺癌患者と対照群の間でのIL-1α, IL-1β産生量は有意差を認めなかった

The activities of interleukin-1 alpha (IL-1 alpha) and interleukin-1 beta) were investigated in peripheral whole blood from 30 patients with bladder cancer, 12 patients with renal cell carcinoma, 18 patients with prostatic cancer and 16 healthy subjects. Heparinized blood was cultured in the absence and presence of various concentrations of bacterial lipopolysaccharide (LPS). The culture supernatants were obtained and activities of IL-1 alpha and IL-1 beta were determined by enzyme-linked immunosorbent assay (ELISA). In the absence of LPS stimulation, neither IL-1 alpha nor IL-1 beta was spontaneously produced in blood cultures from patients with bladder cancer, renal cell carcinoma or prostatic cancer compared with control subjects. After stimulation with various concentrations of LPS, blood cultures from patients with bladder cancer, renal cell carcinoma, prostatic cancer, those from control subjects produced IL-1 alpha and IL-1 beta in a dose-dependent manner, and IL-1 beta was predominant in all supernatants. The activities of IL-1 alpha and IL-1 beta showed no significant differences between the patients with bladder cancer, renal cell carcinoma or prostatic cancer and control subjects. This study suggested that the patients with bladder cancer renal cell carcinoma and prostatic cancer did not spontaneously produce IL-1 alpha or IL-1 beta, but that the ability to produce IL-1 alpha and IL-1 beta in response to LPS stimulation was not significantly impaired.