ヒト腎細胞癌の基礎的研究 第5報 : ヒト腎細胞癌に由来する細胞株OUR-10の性質について

Abstract

A cell line, designated as OUR-10, was established from a human renal cell carcinoma. This cell line have been maintained for more than 28 months with a passage of I week interval. Under inverted microscopy, confluent monolayer of the OUR-10 was composed mainly with polygonal epithelial cells with small round cells or dendritic cells and characterized by the abscence of contact inhibition. With electron microscopy, differentiated cell surface structure looking like microvilli, which was a characteristic fine structure of the human renal cell carcinoma cells, were cbserved. Karyological analysis revealed hypodiploid modal chromosome numbers of 39 and 40. Marker chromosomes with obvious structural anomalies were not detected but non-random loss of three chromosomes in Group D and one in Group E were recognized. The doubling time was approximately 32 hours at the 50th generation. The cells could not form proliferating nodules in the back of the nude mice, even pretreated with cyclophosphamide or X-ray irradiation. But heterotransplantation into the cheek pouch of immunosuppressed hamsters formed tumors, which showed impressive similarity to the original cancer on histology. To clarify the origin of the cell line, several enzymological studies were performed and the T-GTP isozyme contained in the cell line was confirmed to be the same with the novel isozyme detected in about a half of the renal carcinoma tissues. Genetic phenotype of the G6PD was identified as B, and the HLA antigenic structure was A2, II; B5, 40, which were clearly different from the characters of HeLa cells.