Ureaplasma urealyticum精液感染症にかんする研究

Abstract

Some authors have reported that Ureaplama ureal;, ticum is related to male infertility. To answer this question, following studies were conducted. New solid medium, designated modified A3 medium, was prepared in order to perform rapid and steady isolation and identification for Ureaplasma urealyticum from human semen. It consisted of 2.4% trypticase soy broth, 0.9% purified agar, 20% horse serum and 0.25% yeast extract with addition of 1 % urea and 0.02% phenol red. The medium was adjusted to pH 6.0±0.5 and contained 1000 units of penicillin G per milliliter. Positive cultures were obtained from 59% of 53 patients with idiopathic male infertility with the modified A3 medium, 53% with Taylor-Robinson's liquid medium and 51% with Shepards'A3 solid medium. Culture proved positive within 24 hours in 87% of the positive cultures with the modified A3 medium, 46.5% with the liquid medium and 26% with the A3 medium. Semen yielded Ureaplasma urealyticum in 48.6% of 181 patients including the above-mentioned 53 patients. There were no significant differences in semen analyses including sperm count and motility rate among patients with positive or negative cultures. No significant differences were also noted in pH of semen, urea nitrogen level, and IgA and IgG concentrations in seminal plasma. Minocycline was used for treatment of Ureaplasma infection. Seminal plasma levels of minocycline ranged from 1.56 to 2.35 fig/ml, about two thirds of serum level, in 5 patients about 5 to 6 hours later to the last dose of oral administration of 200 mg a day for one week followed by 100 mg a day for 3 weeks. These values were almost the same as the levels obtained in 2 Ureaplasma-negative healthy volunteers. A significant improvement in semen analyses was not found in 28 patients who were successfully treated for Ureaplasma infection.

Some authors have reported that Ureaplama urealyticum is related to male infertility. To answer this question, following studies were conducted. New solid medium, designated modified A3 medium, was prepared in order to perform rapid and steady isolation and identification for Ureaplasma urealyticum from human semen. It consisted of 2.4% trypticase soy broth, 0.9% purified agar, 20% horse serum and 0.25% yeast extract with addition of 1 % urea and 0.02% phenol red. The medium was adjusted to pH 6.0±0.5 and contained 1000 units of penicillin G per milliliter. Positive cultures were obtained from 59% of 53 patients with idiopathic male infertility with the modified A3 medium, 53% with Taylor-Robinson's liquid medium and 51% with Shepards'A3 solid medium. Culture proved positive within 24 hours in 87% of the positive cultures with the modified A3 medium, 46.5% with the liquid medium and 26% with the A3 medium. Semen yielded Ureaplasma urealyticum in 48.6% of 181 patients including the above-mentioned 53 patients. There were no significant differences in semen analyses including sperm count and motility rate among patients with positive or negative cultures. No significant differences were also noted in pH of semen, urea nitrogen level, and IgA and IgG concentrations in seminal plasma. Minocycline was used for treatment of Ureaplasma infection. Seminal plasma levels of minocycline ranged from 1.56 to 2.35 µg/ml, about two thirds of serum level, in 5 patients about 5 to 6 hours later to the last dose of oral administration of 200 mg a day for one week followed by 100 mg a day for 3 weeks. These values were almost the same as the levels obtained in 2 Ureaplasma-negative healthy volunteers. A significant improvement in semen analyses was not found in 28 patients who were successfully treated for Ureaplasma infection.