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Title: Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.
Authors: Takahashi, Kazutoshi kyouindb researcher_resolver
Yamanaka, Shinya kyouindb researcher_resolver
Author's alias: 高橋, 和利
山中, 伸弥
Issue Date: 25-Aug-2006
Publisher: Elsevier Inc.
Journal title: Cell
Volume: 126
Issue: 4
Start page: 663
End page: 676
Abstract: Differentiated cells can be reprogrammed to an embryonic-like state by transfer of nuclear contents into oocytes or by fusion with embryonic stem (ES) cells. Little is known about factors that induce this reprogramming. Here, we demonstrate induction of pluripotent stem cells from mouse embryonic or adult fibroblasts by introducing four factors, Oct3/4, Sox2, c-Myc, and Klf4, under ES cell culture conditions. Unexpectedly, Nanog was dispensable. These cells, which we designated iPS (induced pluripotent stem) cells, exhibit the morphology and growth properties of ES cells and express ES cell marker genes. Subcutaneous transplantation of iPS cells into nude mice resulted in tumors containing a variety of tissues from all three germ layers. Following injection into blastocysts, iPS cells contributed to mouse embryonic development. These data demonstrate that pluripotent stem cells can be directly generated from fibroblast cultures by the addition of only a few defined factors.
Description: This article is related to the Nobel Prize in Physiology or Medicine 2012
Rights: Copyright © 2006 Elsevier Inc. All rights reserved.
This posting is granted exceptional permission by the owner of copyright.
URI: http://hdl.handle.net/2433/159777
DOI(Published Version): 10.1016/j.cell.2006.07.024
PubMed ID: 16904174
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