DSpace Kyoto University
Japanese | English 

Kyoto University Research Information Repository >
Center for iPS Cell Research and Application >
Journal Articles >

Access count of this item: 25995

Please use this identifier to cite or link to this item: http://hdl.handle.net/2433/159777

Full text link:

File Description SizeFormat
j.cell.2006.07.024.pdf1.27 MBAdobe PDFView/Open
Title: Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.
Authors: Takahashi, Kazutoshi kyouindb researcher_resolver
Yamanaka, Shinya kyouindb researcher_resolver
Author's alias: 高橋, 和利
山中, 伸弥
Issue Date: 25-Aug-2006
Publisher: Elsevier Inc.
Journal title: Cell
Volume: 126
Issue: 4
Start page: 663
End page: 676
Abstract: Differentiated cells can be reprogrammed to an embryonic-like state by transfer of nuclear contents into oocytes or by fusion with embryonic stem (ES) cells. Little is known about factors that induce this reprogramming. Here, we demonstrate induction of pluripotent stem cells from mouse embryonic or adult fibroblasts by introducing four factors, Oct3/4, Sox2, c-Myc, and Klf4, under ES cell culture conditions. Unexpectedly, Nanog was dispensable. These cells, which we designated iPS (induced pluripotent stem) cells, exhibit the morphology and growth properties of ES cells and express ES cell marker genes. Subcutaneous transplantation of iPS cells into nude mice resulted in tumors containing a variety of tissues from all three germ layers. Following injection into blastocysts, iPS cells contributed to mouse embryonic development. These data demonstrate that pluripotent stem cells can be directly generated from fibroblast cultures by the addition of only a few defined factors.
Description: This article is related to the Nobel Prize in Physiology or Medicine 2012
Rights: Copyright © 2006 Elsevier Inc. All rights reserved.
This posting is granted exceptional permission by the owner of copyright.
URI: http://hdl.handle.net/2433/159777
DOI(Published Version): 10.1016/j.cell.2006.07.024
PubMed ID: 16904174
Appears in Collections:Journal Articles


Export to RefWorks

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

 

Powered by DSpace 3.2.0 and JAIRO Crawler-List version 1.1
All items in KURENAI are protected by original copyright, with all rights reserved, unless otherwise indicated.
Feedback