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j.celrep.2013.09.016.pdf | 1.06 MB | Adobe PDF | 見る/開く |
タイトル: | Global Splicing Pattern Reversion during Somatic Cell Reprogramming |
著者: | Ohta, Sho Nishida, Eisuke Yamanaka, Shinya Yamamoto, Takuya https://orcid.org/0000-0002-0022-3947 (unconfirmed) |
著者名の別形: | 太田, 翔 西田, 栄介 山中, 伸弥 山本, 拓也 |
発行日: | 17-Oct-2013 |
出版者: | Elsevier |
誌名: | Cell Reports |
開始ページ: | 1 |
終了ページ: | 10 |
抄録: | Alternative splicing generates multiple transcripts from a single gene, and cell-type-specific splicing profiles are important for the properties and functions of the cells. Recently, somatic cells have been shown to undergo dedifferentiation after the forced expression of transcription factors. However, it remains unclear whether somatic cell splicing is reorganized during reprogramming. Here, by combining deep sequencing with high-throughput absolute qRT-PCR, we show that somatic splicing profiles revert to pluripotent ones during reprogramming. Remarkably, the splicing pattern in pluripotent stem cells resembles that in testes, and the regulatory regions have specific characteristics in length and sequence. Furthermore, our siRNA screen has identified RNA-binding proteins that regulate splicing events in iPSCs. We have then demonstrated that two of the RNA-binding proteins, U2af1 and Srsf3, play a role in somatic cell reprogramming. Our results indicate that the drastic alteration in splicing represents part of the molecular network involved in the reprogramming process. |
記述: | 大規模遺伝子解析により体細胞の初期化過程でRNAスプライシングパターンが変化することを解明. 京都大学プレスリリース. 2013-10-18. |
著作権等: | c 2013 The Authors This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-No Derivative Works License, which permits non-commercial use, distribution, and reproduction in any medium, provided the original author and source are credited. |
URI: | http://hdl.handle.net/2433/179283 |
DOI(出版社版): | 10.1016/j.celrep.2013.09.016 |
PubMed ID: | 24139801 |
関連リンク: | https://www.kyoto-u.ac.jp/static/ja/news_data/h/h1/news6/2013_1/131018_1.htm |
出現コレクション: | 学術雑誌掲載論文等 |
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