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タイトル: | Telomere Visualization in Tissue Sections using Pyrrole–Imidazole Polyamide Probes |
著者: | Sasaki, Asuka Ide, Satoru Kawamoto, Yusuke https://orcid.org/0000-0001-9655-8241 (unconfirmed) Bando, Toshikazu Murata, Yukinori Shimura, Mari Yamada, Kazuhiko Hirata, Akiyoshi Nokihara, Kiyoshi Hirata, Tatsumi Sugiyama, Hiroshi https://orcid.org/0000-0001-8923-5946 (unconfirmed) Maeshima, Kazuhiro |
著者名の別形: | 佐々木, 飛鳥 井手, 聖 河本, 佑介 板東, 俊和 村田, 行則 志村, まり 山田, 和彦 平田, 晃義 軒原, 清史 平田, たつみ 杉山, 弘 前島, 一博 |
発行日: | 6-Jul-2016 |
出版者: | Springer Nature |
誌名: | Scientific Reports |
巻: | 6 |
論文番号: | 29261 |
抄録: | Pyrrole–Imidazole (PI) polyamides bind to specific DNA sequences in the minor groove with high affinity. Specific DNA labeling by PI polyamides does not require DNA denaturation with harsh treatments of heat and formamide and has the advantages of rapid and less disruptive processing. Previously, we developed tandem hairpin PI polyamide probes (TH59 series), which label telomeres in cultured cell lines more efficiently than conventional methods, such as fluorescence in situ hybridization (FISH). Here, we demonstrate that a TH59 derivative, HPTH59-b, along with immunostaining for specifying cell types in the tissues, visualizes telomeres in mouse and human tissue sections. Quantitative measurements of telomere length with single-cell resolution suggested shorter telomeres in the proliferating cell fractions of tumor than in non-tumor tissues. Thus, PI polyamides are a promising alternative for telomere labeling in clinical research, as well as in cell biology. |
記述: | さまざまな組織切片の染色体テロメアの長さを3時間で検出できる方法を開発. 京都大学プレスリリース. 2016-07-06. |
著作権等: | This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
URI: | http://hdl.handle.net/2433/215878 |
DOI(出版社版): | 10.1038/srep29261 |
PubMed ID: | 27380936 |
関連リンク: | https://www.kyoto-u.ac.jp/ja/research-news/2016-07-06-1 |
出現コレクション: | 学術雑誌掲載論文等 |
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