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タイトル: | Patient-specific human induced pluripotent stem cell model assessed with electrical pacing validates S107 as a potential therapeutic agent for catecholaminergic polymorphic ventricular tachycardia |
著者: | Sasaki, Kenichi Makiyama, Takeru https://orcid.org/0000-0002-9471-5335 (unconfirmed) Yoshida, Yoshinori https://orcid.org/0000-0001-5511-9090 (unconfirmed) Wuriyanghai, Yimin Kamakura, Tsukasa Nishiuchi, Suguru Hayano, Mamoru Harita, Takeshi Yamamoto, Yuta Kohjitani, Hirohiko https://orcid.org/0000-0002-8281-8396 (unconfirmed) Hirose, Sayako Chen, Jiarong Kawamura, Mihoko Ohno, Seiko Itoh, Hideki Takeuchi, Ayako Matsuoka, Satoshi Miura, Masaru Sumitomo, Naokata Horie, Minoru Yamanaka, Shinya Kimura, Takeshi |
著者名の別形: | 牧山, 武 吉田, 善紀 |
発行日: | 20-Oct-2016 |
出版者: | Public Library of Science |
誌名: | PLOS ONE |
巻: | 11 |
号: | 10 |
論文番号: | e0164795 |
抄録: | Introduction Human induced pluripotent stem cells (hiPSCs) offer a unique opportunity for disease modeling. However, it is not invariably successful to recapitulate the disease phenotype because of the immaturity of hiPSC-derived cardiomyocytes (hiPSC-CMs). The purpose of this study was to establish and analyze iPSC-based model of catecholaminergic polymorphic ventricular tachycardia (CPVT), which is characterized by adrenergically mediated lethal arrhythmias, more precisely using electrical pacing that could promote the development of new pharmacotherapies. Method and Results We generated hiPSCs from a 37-year-old CPVT patient and differentiated them into cardiomyocytes. Under spontaneous beating conditions, no significant difference was found in the timing irregularity of spontaneous Ca2+ transients between control- and CPVT-hiPSCCMs. Using Ca2+ imaging at 1 Hz electrical field stimulation, isoproterenol induced an abnormal diastolic Ca2+ increase more frequently in CPVT- than in control-hiPSC-CMs (control 12% vs. CPVT 43%, p<0.05). Action potential recordings of spontaneous beating hiPSC-CMs revealed no significant difference in the frequency of delayed afterdepolarizations (DADs) between control and CPVT cells. After isoproterenol application with pacing at 1 Hz, 87.5% of CPVT-hiPSC-CMs developed DADs, compared to 30% of control-hiPSCCMs (p<0.05). Pre-incubation with 10 μM S107, which stabilizes the closed state of the ryanodine receptor 2, significantly decreased the percentage of CPVT-hiPSC-CMs presenting DADs to 25% (p<0.05). Conclusions We recapitulated the electrophysiological features of CPVT-derived hiPSC-CMs using electrical pacing. The development of DADs in the presence of isoproterenol was significantly suppressed by S107. Our model provides a promising platform to study disease mechanisms and screen drugs. |
著作権等: | © 2016 Sasaki et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
URI: | http://hdl.handle.net/2433/217229 |
DOI(出版社版): | 10.1371/journal.pone.0164795 |
PubMed ID: | 27764147 |
出現コレクション: | 学術雑誌掲載論文等 |
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