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Title: Integrin α5β1 expression on dopaminergic neurons is involved in dopaminergic neurite outgrowth on striatal neurons
Authors: Izumi, Yasuhiko
Wakita, Seiko
Kanbara, Chisato
Nakai, Toshie
Akaike, Akinori
Kume, Toshiaki
Author's alias: 泉, 安彦
脇田, 誓子
神原, 知里
中井, 利恵
赤池, 昭紀
久米, 利明
Issue Date: 8-Feb-2017
Publisher: Springer Nature
Journal title: Scientific Reports
Volume: 7
Thesis number: 42111
Abstract: During development, dopaminergic neurons born in the substantia nigra extend their axons toward the striatum. However, the mechanisms by which the dopaminergic axons extend the striatum to innervate their targets remain unclear. We previously showed that paired-cultivation of mesencephalic cells containing dopaminergic neurons with striatal cells leads to the extension of dopaminergic neurites from the mesencephalic cell region to the striatal cell region. The present study shows that dopaminergic neurites extended along striatal neurons in the paired-cultures of mesencephalic cells with striatal cells. The extension of dopaminergic neurites was suppressed by the pharmacological inhibition of integrin α5β1. Using lentiviral vectors, short hairpin RNA (shRNA)-mediated knockdown of integrin α5 in dopaminergic neurons suppressed the neurite outgrowth to the striatal cell region. In contrast, the knockdown of integrin α5 in non-dopaminergic mesencephalic and striatal cells had no effect. Furthermore, overexpression of integrin α5 in dopaminergic neurons differentiated from embryonic stem cells enhanced their neurite outgrowth on striatal cells. These results indicate that integrin α5β1 expression on dopaminergic neurons plays an important role in the dopaminergic neurite outgrowth on striatal neurons.
Description: 神経突起が標的神経細胞と相互作用して伸長する仕組みを解明 : 神経細胞移植の治療効果向上に期待. 京都大学プレスリリース. 2017-02-09.
Rights: © The Author(s) 2017. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
URI: http://hdl.handle.net/2433/218040
DOI(Published Version): 10.1038/srep42111
Appears in Collections:Journal Articles


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