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journal.pone.0170342.pdf | 3.54 MB | Adobe PDF | 見る/開く |
タイトル: | SOX10-Nano-Lantern Reporter Human iPS Cells; A Versatile Tool for Neural Crest Research |
著者: | Horikiri, Tomoko Ohi, Hiromi Shibata, Mitsuaki Ikeya, Makoto https://orcid.org/0000-0002-3930-8032 (unconfirmed) Ueno, Morio Sotozono, Chie Kinoshita, Shigeru Sato, Takahiko |
著者名の別形: | 池谷, 真 |
発行日: | 20-Jan-2017 |
出版者: | Public Library of Science |
誌名: | PLOS ONE |
巻: | 12 |
号: | 1 |
論文番号: | e0170342 |
抄録: | The neural crest is a source to produce multipotent neural crest stem cells that have a potential to differentiate into diverse cell types. The transcription factor SOX10 is expressed through early neural crest progenitors and stem cells in vertebrates. Here we report the generation of SOX10-Nano-lantern (NL) reporter human induced pluripotent stem cells (hiPS) by using CRISPR/Cas9 systems, that are beneficial to investigate the generation and maintenance of neural crest progenitor cells. SOX10-NL positive cells are produced transiently from hiPS cells by treatment with TGFβ inhibitor SB431542 and GSK3 inhibitor CHIR99021. We found that all SOX10-NL-positive cells expressed an early neural crest marker NGFR, however SOX10-NL-positive cells purified from differentiated hiPS cells progressively attenuate their NL-expression under proliferation. We therefore attempted to maintain SOX10-NL-positive cells with additional signaling on the plane and sphere culture conditions. These SOX10-NL cells provide us to investigate mass culture with neural crest cells for stem cell research. |
著作権等: | © 2017 Horikiri et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
URI: | http://hdl.handle.net/2433/218475 |
DOI(出版社版): | 10.1371/journal.pone.0170342 |
PubMed ID: | 28107504 |
出現コレクション: | 学術雑誌掲載論文等 |
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