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タイトル: | Angiogenesis in the Developing Spinal Cord: Blood Vessel Exclusion from Neural Progenitor Region Is Mediated by VEGF and Its Antagonists |
著者: | Takahashi, Teruaki Takase, Yuta Yoshino, Takashi Saito, Daisuke Tadokoro, Ryosuke Takahashi, Yoshiko https://orcid.org/0000-0002-1596-7527 (unconfirmed) |
著者名の別形: | 高橋, 淑子 |
キーワード: | Biochemistry, Genetics and Molecular Biology(all) Agricultural and Biological Sciences(all) Medicine(all) |
発行日: | 13-Jan-2015 |
出版者: | Public Library of Science (PLoS) |
誌名: | PLOS ONE |
巻: | 10 |
号: | 1 |
論文番号: | e0116119 |
抄録: | Blood vessels in the central nervous system supply a considerable amount of oxygen via intricate vascular networks. We studied how the initial vasculature of the spinal cord is formed in avian (chicken and quail) embryos. Vascular formation in the spinal cord starts by the ingression of intra-neural vascular plexus (INVP) from the peri-neural vascular plexus (PNVP) that envelops the neural tube. At the ventral region of the PNVP, the INVP grows dorsally in the neural tube, and we observed that these vessels followed the defined path at the interface between the medially positioned and undifferentiated neural progenitor zone and the laterally positioned differentiated zone. When the interface between these two zones was experimentally displaced, INVP faithfully followed a newly formed interface, suggesting that the growth path of the INVP is determined by surrounding neural cells. The progenitor zone expressed mRNA of vascular endothelial growth factor-A whereas its receptor VEGFR2 and FLT-1 (VEGFR1), a decoy for VEGF, were expressed in INVP. By manipulating the neural tube with either VEGF or the soluble form of FLT-1, we found that INVP grew in a VEGF-dependent manner, where VEGF signals appear to be fine-tuned by counteractions with anti-angiogenic activities including FLT-1 and possibly semaphorins. These results suggest that the stereotypic patterning of early INVP is achieved by interactions between these vessels and their surrounding neural cells, where VEGF and its antagonists play important roles. |
著作権等: | © 2015 Takahashi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited |
URI: | http://hdl.handle.net/2433/219096 |
DOI(出版社版): | 10.1371/journal.pone.0116119 |
PubMed ID: | 25585380 |
出現コレクション: | 学術雑誌掲載論文等 |
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