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タイトル: | Tim4- and MerTK-mediated engulfment of apoptotic cells by mouse resident peritoneal macrophages. |
著者: | Nishi, Chihiro Toda, Satoshi Segawa, Katsumori Nagata, Shigekazu |
著者名の別形: | 長田, 重一 |
発行日: | Apr-2014 |
出版者: | American Society for Microbiology |
誌名: | Molecular and cellular biology |
巻: | 34 |
号: | 8 |
開始ページ: | 1512 |
終了ページ: | 1520 |
抄録: | Apoptotic cells are swiftly engulfed by macrophages to prevent the release of noxious materials from dying cells. Apoptotic cells expose phosphatidylserine (PtdSer) on their surface, and macrophages engulf them by recognizing PtdSer using specific receptors and opsonins. Here, we found that mouse resident peritoneal macrophages expressing Tim4 and MerTK are highly efficient at engulfing apoptotic cells. Neutralizing antibodies against either Tim4 or MerTK inhibited the macrophage engulfment of apoptotic cells. Tim4-null macrophages exhibited reduced binding and engulfment of apoptotic cells, whereas MerTK-null macrophages retained the ability to bind apoptotic cells but failed to engulf them. The incubation of wild-type peritoneal macrophages with apoptotic cells induced the rapid tyrosine phosphorylation of MerTK, which was not observed with Tim4-null macrophages. When mouse Ba/F3 cells were transformed with Tim4, apoptotic cells bound to the transformants but were not engulfed. Transformation of Ba/F3 cells with MerTK had no effect on the binding or engulfment of apoptotic cells; however, Tim4/MerTK transformants exhibited strong engulfment activity. Taken together, these results indicate that the engulfment of apoptotic cells by resident peritoneal macrophages proceeds in two steps: binding to Tim4, a PtdSer receptor, followed by MerTK-mediated cell engulfment. |
著作権等: | © 2014, American Society for Microbiology. This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 |
URI: | http://hdl.handle.net/2433/189107 |
DOI(出版社版): | 10.1128/MCB.01394-13 |
PubMed ID: | 24515440 |
出現コレクション: | 学術雑誌掲載論文等 |
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