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dc.contributor.author | Kaneko, Takehito | en |
dc.contributor.author | Ito, Hideyuki | en |
dc.contributor.author | Sakamoto, Hidefusa | en |
dc.contributor.author | Onuma, Manabu | en |
dc.contributor.author | Inoue-Murayama, Miho | en |
dc.contributor.alternative | 金子, 武人 | ja |
dc.date.accessioned | 2014-12-25T07:53:48Z | - |
dc.date.available | 2014-12-25T07:53:48Z | - |
dc.date.issued | 2014-11-19 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | http://hdl.handle.net/2433/192770 | - |
dc.description.abstract | Sperm preservation is a useful technique for the maintenance of biological resources in experimental and domestic animals, and in wild animals. A new preservation method has been developed that enables sperm to be stored for a long time in a refrigerator at 4°C. Sperm are freeze-dried in a solution containing 10 mM Tris and 1 mM EDTA. Using this method, liquid nitrogen is not required for the storage and transportation of sperm. We demonstrate that chimpanzee, giraffe, jaguar, weasel and the long-haired rat sperm remain viable after freeze-drying. In all species, pronuclei were formed after the injection of freeze-dried sperm into the mouse oocytes. Although preliminary, these results may be useful for the future establishment of "freeze-drying zoo" to conserve wild animals. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Public Library of Science | en |
dc.rights | © 2014 Kaneko et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | en |
dc.title | Sperm preservation by freeze-drying for the conservation of wild animals. | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.jtitle | PloS one | en |
dc.identifier.volume | 9 | - |
dc.identifier.issue | 11 | - |
dc.relation.doi | 10.1371/journal.pone.0113381 | - |
dc.textversion | publisher | - |
dc.identifier.artnum | e113381 | - |
dc.identifier.pmid | 25409172 | - |
dcterms.accessRights | open access | - |
出現コレクション: | 学術雑誌掲載論文等 |
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