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dc.contributor.author | Shintaku, Hirofumi | en |
dc.contributor.author | Palko, James W. | en |
dc.contributor.author | Sanders, Glenn M. | en |
dc.contributor.author | Santiago, Juan G. | en |
dc.contributor.alternative | 新宅, 博文 | ja |
dc.date.accessioned | 2015-08-27T04:54:54Z | - |
dc.date.available | 2015-08-27T04:54:54Z | - |
dc.date.issued | 2014-10-10 | - |
dc.identifier.issn | 1433-7851 | - |
dc.identifier.uri | http://hdl.handle.net/2433/199656 | - |
dc.description | マイクロ流体技術を用いた網羅的・高速DNA分析技術の開発に成功 -網羅的・短時間・高感度のバイオマーカ検出技術の確立に期待-. 京都大学プレスリリース. 2014-10-15. | ja |
dc.description.abstract | We present an electrokinetic technique to increase the reaction rate and sensitivity of bead-based assays. We use isotachophoresis (ITP) to preconcentrate and co-focus target molecules and beads into a single ITP zone. The process achieves rapid mixing, stirring, and strongly increases the binding reaction rate. We demonstrate our assay with quantitative detection of 24 nt single-stranded DNA over a dynamic range of three orders of magnitude and multiplexed detection of ten target species per sample. We show that ITP can achieve approximately the same sensitivity as a well-stirred standard reaction in 60-fold reduced reaction time (20 min versus 20 h). Alternately, compared to standard reaction times of 30 min, we show that 20 min ITP hybridization can achieve 5.3-fold higher sensitivity. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | wiley | en |
dc.rights | This is the peer reviewed version of the following article: Shintaku, H., Palko, J. W., Sanders, G. M. and Santiago, J. G. (2014), Increasing Hybridization Rate and Sensitivity of Bead-Based Assays Using Isotachophoresis. Angew. Chem., 126: 14033–14036, which has been published in final form at http://dx.doi.org/10.1002/ange.201408403. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. | en |
dc.rights | 許諾条件により本文ファイルは2015-10-10に公開. | ja |
dc.rights | この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 | ja |
dc.rights | This is not the published version. Please cite only the published version. | en |
dc.subject | DNA-Analyse | en |
dc.subject | Hybridisierungsassay | en |
dc.subject | Isotachophorese | en |
dc.subject | Paralleldetektion | en |
dc.title | Increasing Hybridization Rate and Sensitivity of Bead-Based Assays Using Isotachophoresis | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.ncid | AA0052535X | - |
dc.identifier.jtitle | Angewandte Chemie | en |
dc.identifier.volume | 126 | - |
dc.identifier.issue | 50 | - |
dc.identifier.spage | 14033 | - |
dc.identifier.epage | 14036 | - |
dc.relation.doi | 10.1002/ange.201408403 | - |
dc.textversion | author | - |
dc.startdate.bitstreamsavailable | 2015-10-10 | - |
dc.relation.url | https://www.kyoto-u.ac.jp/ja/research-news/2014-10-15 | - |
dcterms.accessRights | open access | - |
出現コレクション: | 学術雑誌掲載論文等 |
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