ダウンロード数: 365
このアイテムのファイル:
ファイル | 記述 | サイズ | フォーマット | |
---|---|---|---|---|
15376516.2013.876136.pdf | 334.17 kB | Adobe PDF | 見る/開く |
タイトル: | Effects of hydrogen peroxide on mucociliary transport in human airway epithelial cells. |
著者: | Honda, Akiko https://orcid.org/0000-0002-7358-4372 (unconfirmed) Murayama, Rumiko Matsuda, Yugo Tsuji, Kenshi Sawahara, Takahiro Fukushima, Wataru Hayashi, Tomohiro Shimada, Akinori Takano, Hirohisa |
著者名の別形: | 本田, 晶子 |
キーワード: | Air–liquid interface culture airway epithelial cells hydrogen peroxide mucociliary transport |
発行日: | 13-Jan-2014 |
出版者: | Taylor & Francis Group |
誌名: | Toxicology mechanisms and methods |
巻: | 24 |
号: | 3 |
開始ページ: | 191 |
終了ページ: | 195 |
抄録: | The effects of environmental pollutants on airway clearance have not been well elucidated. This study examined mucociliary transport using different sized-fluorescent particles on polarized human airway epithelial cells which were maintained in an air-liquid interface (ALI) culture system. The effects of hydrogen peroxide (H2O2) exposure on mucociliary transport were also investigated. The movement of fluorescent particles with diameters of 10-14 and 2.5-4.5 µm was observed by fluorescent microscopy as an index of the mucociliary transport. The mixture of the particles with two different sizes was propelled concentrically on the apical surface by the interaction of ciliary activity and mucus in the control condition, whereas H2O2 exposure for 24 h significantly inhibited the movement of the particles. The particle sizes did not affect their movement after the control or H2O2 exposure. These results suggest that particle tracking on polarized human airway epithelial cells is a useful experimental tool for the evaluation of the effect of environmental pollutants on mucociliary transport. In addition, reactive oxygen species may impair mucociliary transport, leading to the airway damage and exacerbation of respiratory diseases. |
著作権等: | This is an Accepted Manuscript of an article published by Taylor & Francis in "Toxicology Mechanisms and Methods" on Published online: 13 Jan 2014, available online: http://www.tandfonline.com/10.3109/15376516.2013.876136. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 This is not the published version. Please cite only the published version. |
URI: | http://hdl.handle.net/2433/199890 |
DOI(出版社版): | 10.3109/15376516.2013.876136 |
PubMed ID: | 24354798 |
出現コレクション: | 学術雑誌掲載論文等 |
このリポジトリに保管されているアイテムはすべて著作権により保護されています。