ダウンロード数: 623
このアイテムのファイル:
ファイル | 記述 | サイズ | フォーマット | |
---|---|---|---|---|
nature14909.pdf | 23.42 MB | Adobe PDF | 見る/開く |
タイトル: | Structure and mechanism of the mammalian fructose transporter GLUT5. |
著者: | Nomura, Norimichi https://orcid.org/0000-0002-6330-2239 (unconfirmed) Verdon, Grégory Kang, Hae Joo Shimamura, Tatsuro Nomura, Yayoi Sonoda, Yo Hussien, Saba Abdul Qureshi, Aziz Abdul Coincon, Mathieu Sato, Yumi Abe, Hitomi Nakada-Nakura, Yoshiko Hino, Tomoya Arakawa, Takatoshi Kusano-Arai, Osamu Iwanari, Hiroko Murata, Takeshi Kobayashi, Takuya Hamakubo, Takao Kasahara, Michihiro Iwata, So Drew, David |
著者名の別形: | 野村, 紀通 岩田, 想 |
発行日: | 30-Sep-2015 |
出版者: | Nature Publishing Group |
誌名: | Nature |
巻: | 526 |
号: | 7573 |
開始ページ: | 397 |
終了ページ: | 401 |
抄録: | The altered activity of the fructose transporter GLUT5, an isoform of the facilitated-diffusion glucose transporter family, has been linked to disorders such as type 2 diabetes and obesity. GLUT5 is also overexpressed in certain tumour cells, and inhibitors are potential drugs for these conditions. Here we describe the crystal structures of GLUT5 from Rattus norvegicus and Bos taurus in open outward- and open inward-facing conformations, respectively. GLUT5 has a major facilitator superfamily fold like other homologous monosaccharide transporters. On the basis of a comparison of the inward-facing structures of GLUT5 and human GLUT1, a ubiquitous glucose transporter, we show that a single point mutation is enough to switch the substrate-binding preference of GLUT5 from fructose to glucose. A comparison of the substrate-free structures of GLUT5 with occluded substrate-bound structures of Escherichia coli XylE suggests that, in addition to global rocker-switch-like re-orientation of the bundles, local asymmetric rearrangements of carboxy-terminal transmembrane bundle helices TM7 and TM10 underlie a 'gated-pore' transport mechanism in such monosaccharide transporters. |
記述: | 糖分を細胞内に輸送する膜たんぱく質の立体構造と動きを解明 -肥満やがんの抑制策に役立つ新たな知見-. 京都大学プレスリリース. 2015-10-01. |
著作権等: | © 2015 Macmillan Publishers Limited. The full-text file will be made open to the public on 30 march 2016 in accordance with publisher's 'Terms and Conditions for Self-Archiving'. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 This is not the published version. Please cite only the published version. |
URI: | http://hdl.handle.net/2433/199936 |
DOI(出版社版): | 10.1038/nature14909 |
PubMed ID: | 26416735 |
関連リンク: | https://www.kyoto-u.ac.jp/ja/research-news/2015-10-01 |
出現コレクション: | 学術雑誌掲載論文等 |
このリポジトリに保管されているアイテムはすべて著作権により保護されています。