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dc.contributor.author | Adachi, Shun | en |
dc.contributor.author | Murakawa, Yasuhiro | en |
dc.contributor.author | Hiraga, Sota | en |
dc.contributor.alternative | 足立, 隼 | ja |
dc.contributor.alternative | 村川, 泰裕 | ja |
dc.contributor.alternative | 平賀, 壯太 | ja |
dc.date.accessioned | 2015-10-06T06:39:02Z | - |
dc.date.available | 2015-10-06T06:39:02Z | - |
dc.date.issued | 2014-08-01 | - |
dc.identifier.issn | 1465-2080 | - |
dc.identifier.uri | http://hdl.handle.net/2433/200187 | - |
dc.description.abstract | Spatial regulation of nucleoids and chromosome-partitioning proteins is important for proper chromosome partitioning in Escherichia coli. However, the underlying molecular mechanisms are unknown. In the present work, we showed that mutation or chemical perturbation of secretory A (SecA), an ATPase component of the membrane protein translocation machinery, SecY, a component of the membrane protein translocation channel and acyl carrier protein P (AcpP), which binds to SecA and MukB, a functional homologue of structural maintenance of chromosomes protein (SMC), resulted in a defect in chromosome partitioning. We further showed that SecA is essential for proper positioning of the oriC DNA region, decatenation and maintenance of superhelicity of DNA. Genetic interaction studies revealed that the topological abnormality observed in the secA mutant was due to combined inhibitory effects of defects in MukB, DNA gyrase and Topo IV, suggesting a role for the membrane protein translocation machinery in chromosome partitioning and/or structural maintenance of chromosomes. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Microbiology Society | en |
dc.rights | © 2014 The Authors. Published by the Microbiology Society. | en |
dc.rights | This is not the published version. Please cite only the published version. | en |
dc.rights | この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 | ja |
dc.subject.mesh | Adenosine Triphosphatases/genetics | en |
dc.subject.mesh | Adenosine Triphosphatases/metabolism | en |
dc.subject.mesh | Bacterial Proteins/genetics | en |
dc.subject.mesh | Bacterial Proteins/metabolism | en |
dc.subject.mesh | Chromosomal Proteins, Non-Histone/genetics | en |
dc.subject.mesh | Chromosomal Proteins, Non-Histone/metabolism | en |
dc.subject.mesh | Chromosomes, Bacterial/genetics | en |
dc.subject.mesh | Chromosomes, Bacterial/metabolism | en |
dc.subject.mesh | DNA Gyrase/genetics | en |
dc.subject.mesh | DNA Gyrase/metabolism | en |
dc.subject.mesh | DNA, Bacterial/genetics | en |
dc.subject.mesh | DNA, Bacterial/metabolism | en |
dc.subject.mesh | DNA, Superhelical/genetics | en |
dc.subject.mesh | DNA, Superhelical/metabolism | en |
dc.subject.mesh | Escherichia coli/enzymology | en |
dc.subject.mesh | Escherichia coli/genetics | en |
dc.subject.mesh | Escherichia coli Proteins/genetics | en |
dc.subject.mesh | Escherichia coli Proteins/metabolism | en |
dc.subject.mesh | Gene Expression Regulation, Bacterial | en |
dc.subject.mesh | Membrane Transport Proteins/genetics | en |
dc.subject.mesh | Membrane Transport Proteins/metabolism | en |
dc.title | SecA defects are accompanied by dysregulation of MukB, DNA gyrase, chromosome partitioning and DNA superhelicity in Escherichia coli. | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.jtitle | Microbiology | en |
dc.identifier.volume | 160 | - |
dc.identifier.spage | 1648 | - |
dc.identifier.epage | 1658 | - |
dc.relation.doi | 10.1099/mic.0.077685-0 | - |
dc.textversion | author | - |
dc.startdate.bitstreamsavailable | 2015-08-15 | - |
dc.identifier.pmid | 24858081 | - |
dcterms.accessRights | open access | - |
出現コレクション: | 学術雑誌掲載論文等 |
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