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| ファイル | 記述 | サイズ | フォーマット | |
|---|---|---|---|---|
| j.dib.2015.09.037.pdf | 721.78 kB | Adobe PDF | 見る/開く |
| タイトル: | Data of a fluorescent imaging-based analysis of anti-cancer drug effects on three-dimensional cultures of breast cancer cells |
| 著者: | Itou, Junji  Tanaka, Sunao Li, Wenzhao Matsumoto, Yoshiaki  https://orcid.org/0000-0002-4256-2349 (unconfirmed)Sato, Fumiaki Toi, Masakazu https://orcid.org/0000-0003-1488-9958 (unconfirmed) |
| 著者名の別形: | 伊東, 潤二 |
| キーワード: | Anti-cancer drug Breast cancer Live imaging Photoconvertible fluorescent protein Pulse labeling Three-dimensional culture |
| 発行日: | Dec-2015 |
| 出版者: | Elsevier Inc. |
| 誌名: | Data in Brief |
| 巻: | 5 |
| 開始ページ: | 429 |
| 終了ページ: | 433 |
| 抄録: | Three-dimensional (3D) cell culture is a powerful tool to study cell growth under 3D condition. To perform a simple test for anti-cancer drugs in 3D culture, visualization of non-proliferated cells is required. We propose a fluorescent imaging-based assay to analyze cancer cell proliferation in 3D culture. We used a pulse-labeling technique with a photoconvertible fluorescent protein Kaede to identify non-proliferated cells. This assay allows us to observe change in cell proliferation in 3D culture by simple imaging. Using this assay, we obtained the data of the effects of anti-cancer drugs, 5-fluorouracil and PD0332991 in a breast cancer cell line, MCF-7. |
| 著作権等: | © 2015 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
| URI: | http://hdl.handle.net/2433/203047 |
| DOI(出版社版): | 10.1016/j.dib.2015.09.037 |
| PubMed ID: | 26958605 |
| 出現コレクション: | 学術雑誌掲載論文等 |
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