ダウンロード数: 869
このアイテムのファイル:
ファイル | 記述 | サイズ | フォーマット | |
---|---|---|---|---|
pros.23088.pdf | 916.13 kB | Adobe PDF | 見る/開く |
タイトル: | Decreased expression of lysophosphatidylcholine (16:0/OH) in high resolution imaging mass spectrometry independently predicts biochemical recurrence after surgical treatment for prostate cancer. |
著者: | Goto, Takayuki Terada, Naoki Inoue, Takahiro Kobayashi, Takashi Nakayama, Kenji Okada, Yoshiyuki Yoshikawa, Takeshi Miyazaki, Yu Uegaki, Masayuki Utsunomiya, Noriaki Makino, Yuki Sumiyoshi, Shinji Yamasaki, Toshinari Kamba, Tomomi Ogawa, Osamu |
著者名の別形: | 後藤, 崇之 小川, 修 |
キーワード: | imaging mass spectrometry lysophosphatidylcholine prostate cancer biomarker lipid |
発行日: | Dec-2015 |
出版者: | wiley |
誌名: | The Prostate |
巻: | 75 |
号: | 16 |
開始ページ: | 1821 |
終了ページ: | 1830 |
抄録: | [Background]Human prostate cancers are highly heterogeneous, indicating a need for various novel biomarkers to predict their prognosis. Lipid metabolism affects numerous cellular processes, including cell growth, proliferation, differentiation, and motility. Direct profiling of lipids in tissue using high-resolution matrix-assisted laser desorption/ionization imaging mass spectrometry (HR-MALDI-IMS) may provide molecular details that supplement tissue morphology. [Methods]Prostate tissue samples were obtained from 31 patients, with localized prostate cancer who underwent radical prostatectomy. The samples were assessed by HR-MALDI-IMS in positive mode, with the molecules identified by tandem mass spectrometry (MS/MS). The effect of identified molecules on prostate specific antigen recurrence free survival after radical prostatectomy was determined by Cox regression analysis and by the Kaplan–Meier method. [Results]Thirteen molecules were found to be highly expressed in prostate tissue, with five being significantly lower in cancer tissue than in benign epithelium. MS/MS showed that these molecules were [lysophosphatidylcholine (LPC)(16:0/OH)+H]+, [LPC(16:0/OH)+Na]+, [LPC(16:0/OH)+K]+, [LPC(16:0/OH)+matrix+H]+, and [sphingomyelin (SM)(d18:1/16:0)+H]+. Reduced expression of LPC(16:0/OH) in cancer tissue was an independent predictor of biochemical recurrence after radical prostatectomy. [Conclusions]HR-MALDI-IMS showed that the expression of LPC(16:0/OH) and SM(d18:1/16:0) was lower in prostate cancer than in benign prostate epithelium. These differences in expression of phospholipids may predict prostate cancer aggressiveness, and provide new insights into lipid metabolism in prostate cancer. |
記述: | Article first published online: 1 SEP 2015 |
著作権等: | This is the accepted version of the following article: [Goto, T., Terada, N., Inoue, T., Kobayashi, T., Nakayama, K., Okada, Y., Yoshikawa, T., Miyazaki, Y., Uegaki, M., Utsunomiya, N., Makino, Y., Sumiyoshi, S., Yamasaki, T., Kamba, T. and Ogawa, O. (2015), Decreased expression of lysophosphatidylcholine (16:0/OH) in high resolution imaging mass spectrometry independently predicts biochemical recurrence after surgical treatment for prostate cancer. Prostate, 75: 1821–1830.], which has been published in final form at http://dx.doi.org/10.1002/pros.23088. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. The full-text file will be made open to the public on 1 September 2016 in accordance with publisher's 'Terms and Conditions for Self-Archiving'. This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 |
URI: | http://hdl.handle.net/2433/204214 |
DOI(出版社版): | 10.1002/pros.23088 |
PubMed ID: | 26332786 |
出現コレクション: | 学術雑誌掲載論文等 |
このリポジトリに保管されているアイテムはすべて著作権により保護されています。