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タイトル: | Selective labeling of proteins on living cell membranes using fluorescent nanodiamond probes |
著者: | Sotoma, Shingo Iimura, Jun Igarashi, Ryuji Hirosawa, Koichiro M. Ohnishi, Hidenori Mizukami, Shin Kikuchi, Kazuya Fujiwara, Takahiro K. Shirakawa, Masahiro Tochio, Hidehito https://orcid.org/0000-0003-3843-3330 (unconfirmed) |
著者名の別形: | 五十嵐, 龍治 藤原, 敬宏 白川, 昌宏 栃尾, 豪人 |
キーワード: | nanodiamond nitrogen-vacancy center polyglycerol β-lactamase tag membrane protein |
発行日: | 25-Mar-2016 |
出版者: | MDPI AG |
誌名: | Nanomaterials |
巻: | 6 |
号: | 4 |
論文番号: | 56 |
抄録: | The impeccable photostability of fluorescent nanodiamonds (FNDs) is an ideal property for use in fluorescence imaging of proteins in living cells. However, such an application requires highly specific labeling of the target proteins with FNDs. Furthermore, the surface of unmodified FNDs tends to adsorb biomolecules nonspecifically, which hinders the reliable targeting of proteins with FNDs. Here, we combined hyperbranched polyglycerol modification of FNDs with the -lactamase-tag system to develop a strategy for selective imaging of the protein of interest in cells. The combination of these techniques enabled site-specific labeling of Interleukin-18 receptor alpha chain, a membrane receptor, with FNDs, which eventually enabled tracking of the diffusion trajectory of FND-labeled proteins on the membrane surface. |
著作権等: | © 2016 by the authors; licensee MDPI, Basel, Switzerland. This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
URI: | http://hdl.handle.net/2433/214443 |
DOI(出版社版): | 10.3390/nano6040056 |
PubMed ID: | 28335184 |
出現コレクション: | 学術雑誌掲載論文等 |
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