腎結核に於けるアミノ酸の研究(ペーパークロマトグラフイーに依る) 第I編:腎結核組織内遊離アミノ酸の分布(臨床実験成績)

Abstract

The partition chromatography designed by M a r tin and Synge, and the paper partition chromatography generalized by Consden, Gordon and Martin in 1949 have caused excellent results in various fields of science. Moreover since the first introduction of the detailed techniques by Hagioka in 1949, many investigations, using this method, have been made. With the cosideration that the correlation, if any, would be found out betwe e n the amount of free amino acids in the tuberculous focus and that in other tissue, many experiments were done for several years by using the paper partition chromatography and revealed the facts described in the following three parts. In this part of the report, the basic experiment to paper partition chromatography and the method adopted by this author are described, and then the free amino acids within the cavity tissue of thirty kidneys extirpated under the diagnosis of kidney tuberculosis are qualitatively distinguished by applying the two dimension method of paper partition chromatography. The following fifteen free amino acids could be easily recognized asparatic acid, glutamic acid, tyrosine, serine, valine, leucine, alanine, threonine, glycine, phenylalanine and arginine, S i x amino acids of cystine, proline, histidine, lysine, phenylalanine and arginine are easily fluctuated when the condition varies. In the ten extirpated tuberculous kidneys the free amino acids contained only in the caseous substance of its kidney were distinctly observed by the same way, and they were serine, glutamic acid, asparatic acid, alanine, glycine, tyrosine, valine, threonine, cystine, phenylanine, proline, leucine, histidine and methionine. The seven amino acids after threonine in the above described amino acids appear in less frequency and change in quantity and quality by the degree of proteolytic process,