泌尿器科領域の蛋白質をめぐる2,3の問題 第1篇:尿石の蛋白質成分の研究

Abstract

Almost all of the components of the urinary calculi ar e inorganic substances. Many urologists, however, were much interested in the organic components of urinary calculi recently. Several kinds of protein in these organic components of uriynary calculi were studied in this paper. 1) M e thode: 0.3- 1.0 gms of powder of urinary calculus was hydrolysated with 20 times of 8n-sulphuric acid (20 cc H2H04/100cc Aq.) in 100°C-110°C and it was neutralized with barium hydroxide followed by filtration. Then the filtrate was concentrated and a little of it was used for paper chromatography with phenol and n-butanol as solvent. 2) Results: The value of each amino-acid estimated in this study was s howed in table 2-9 in many urinary calculi located in kidney, ureter, bladder, prostate gland and vesicovaginal fistula. As shown in these tables, amino-acdis, most of which were aspartic acid, serine, glucosamine, alanine and methionine, were found in almost all of these stones. Al t h ough cystine and valine were found very frequently in upper urinary calculi, glutamic acid was much more found than cystine in lower urinary calculi. Cystinic acid and valine were main components of prostatic stones. A large amounts of serine was. revealed specifically in the nonopaque stone with x-ray. 3) Hydrolystic amino-acids of the pearls : At th e same time a pearl was hydrolysated with 8n-sulphuric acid, many amino-acids were proved as shown in table 10. Hydrolytic amino-acids of the pearl were similar to the case of urinary stones, but the several amino-acids, cystinic acid, cystine, glucosamine and methionine, which were found very freqently in urinary stones were not shown in pearls. 4) Significance of protein in the urinary ca l c uli for the stone formation : It seems to me that the protein is not always necessary for the growth of urinary calculi, but cooperate secondarily with anorganic substances after the study on composition of urinary calculi crystallographically, electro chemically and quantum mechanically. Read at 43rd general meeting of Japan Urological Associa t i on in Kyoto, April, 1955,