膀胱炎に関する研究 第III編:耐性菌及び抗生物質併用療法に関する研究

Abstract

This report deals bacteriological studies on sensit i vity of antibiotic resistant strains to other antibiotics, and on effect of combination of antibiotics. 1) In vitro M. pyog. var. aureus 209P strain b ecame quickly and strongly resistant to streptmycin, and E. coli communior FCR strain became quickly resistant to chloramphenicol. 2) The 209P strain aquired resistance to penicillin in vitro was slightly more sensitive to streptmycin and chloramphenicol than the original strain. The 209P strain aquired resistance to streptmycin was slightly more sensitive to chloramphenicol than the original strain. The FCR strain aqired resistance to streptomycin was slightly more sensitive to chloramphenicol than the original strain and the FCR strain aquired resistance to chlortetracycline was slightly more sensitive to streptomycin. Using 209P strain class resestance o f chlortetracycline wih oxytetracycline and using FCR strain cross resistance of chloramphenical with chior-and oxytetracycline and of chlortetracycline with oxytetracycline were observed. 3) Antibiotic sensitivity test to antibiotic resistant strains containing 56 strains of M. pyog, var. albus and 11 of M. pyog. var. aureus isolated from vesical urine of patients with cystitis showed cross resistance of chlortetracycline with oxytetracycline. The test to antibiotic resistant 17 Coli-type colon bacillus strains showed cross resistance of chloramphenicol with chior-and oxytetracycline and of chlortetracycline with oxytetracycline. All of the M. pyog. var. albus strains resistant to other antibiotics was resistant to penicillin too, all of the M. pyog. var. aureus resistant to chior-and oxytetracycline was resistant to penicillin and streptmycin too and the greater part of these aureus strains resistant to streptomycin was resistant to penicillin too. No double resistance to chloramphen i c ol and chlortetracycline, to chloramphenicol and oxytetracycline, to erythromycin and chlortetracycline and to erythromycin and oxytetracycline was shown by the observation on these M. pyog. var. albus strains. By the observation on these Coli-type strains no double resistance to streptomycin and chlot amphenicol was shown. 4) Oleandomycin revealed activity against M. pyog. var. aureus 209P strains which had become resistant in vitro to penicillin, to streptomycin, to chloramphenicol, to chlortetracycline, to oxytetracycline and to erythromycin, and against clinical strains of staphylococci resistant to these antibiotics. Treatment for acute cystitis with oleandomycin was succesfully performed. 5) Test for inhibitting effect to growth of 209P strain showed potentiative effect between erythromycin and penicillin, chloramphenicol and oxytetracycline and between oleandomycin and penicillin, chloramphenicol and tetracycline, indifferent effect between erythromycin and streptomycin and antagonistic effect between oleandomycin and streptomycin. Test for inhibitting effect to growth of clinical isolated staphylococcus strains showed potentiative effect between erythromycin and oxytetracycline. Test for inhibitting effect to growth of FC R strain showed potentiated effect between erythromycin and oxytetracycline. 6) Treatment f o r acute cystis with combination of erythromycin and oxytetracycline was successfully performed.