泌尿器科領域に於ける17-Ketosteroidの研究補遺 第1篇:17-Ketosteroidの概念並びに測定法

Abstract

1) On the basis of Zimmermann's m-dinitrobenzene reaction, rate of 17-ketosteroid excretion was measured by means of the modified "Drekter's or Pearson's" method, i.e. Miyake's method. 2) In order to study the color reaction, 8N KOH was utilized for Pearson's method which produced better stability and quick responses. 3) The standard crystalline steroids w e re utilized for the test of ketonic androsterone, except for nonketonic substances which was examined by testosterone. 4) As for the color correction, the equation of Fraser was adopted. 5) Hydrolysis and extraction was carried out in the method o f Drekter's and Miyake's.