|Other Titles:||A Studies on Bacterial Flora of Japanese Famale Urethra (Part II) Detective Experiments on Normal Bacterial Flora of Female Urethra with Some Remarks on Competitive Actions among Bacterial Strains|
|Author's alias:||NISHIKAGE, Yuzi|
|Abstract:||1) Aerobic cultivation of bacterial flora of normal 100 female urethra resulted in the detection of 6 strains of gram positive coccus, 2 strains of gram positive rods, 2 strains of gram negative coccus and 2 strains of gram negative rod. It was also demonstrated that almost similar tendencies were found between bacterial flora in vaginal orifice and that in external urethral orifice in respects to the bacterial species as well as the detective rates. These evidences clearly indicate that the flora of external urethral orifice is affected by invasion of secreted fluid of vagina. 2) Gram positive cocci and rods were detecte d and isolated in a high rate from external urethral orifice of normal female subjects ; 68 % of the isolated organisms are Staphylococci, all of which are classified as Staphylococcus epidermidis according to the Bergey's classification. 3) A c c ording to the method of swab isolation from an appointed part on female urethra as mentioned in the previous report I in a series of the studies, aerobic and anerobic cultivation were made with bacteria of 180 normal female urethra. The results indicated that, in 167 out of the 180 subjects, no organism was detected from an internal part of 2.0-3.0c m in the distance from the external urethral orifice. 4) Regarding 100 subjects suffered from urethritis and cy s titis, cultivation was made with bacteria of the vaginal orifice and the external urethral orifice. The results demonstrated that, as in the normal subjects, similar tendencies were found between the bacterial flora of the vaginal orifice and that of the external urethral orifice in respects to the bacterial species as well as the detective rates. It was also demonstrated that gram positive cocci and rods were detected in 18-64 % of all the patients, but the flora count of the gram positive cocci and rods on the pure culture medium was always in low unlikely from the case of normal subjects. On the contrary, it was demonstrated that the detective rate of gram negative cocci and rods was as high as 2 fold in the patients as co mpared with the •-ormal subjects, and that the colonies count on the respective plate medium was found to be higher in gram negative cocci and rods as compared with gram positive cocci and rods. 5) Apart from t he evidences in normal subjects, urethral bacteria was detected from an internal part of 2.0-3.0 cm in the distance from the external urethral orifice in 62-84% of the patients with urethritis and acute cystitis. According to the bacterial species, Stapylococci were detected in a high rate of the patients as in normal subjects, but the detective rate of gram negative cocci was as high as 3-5 fold in a part of 1.0 cm from the distance from the external urethral orifice of the patients as compared with in the similar part of normal subjects. Gram negative rods in urethra were detected in none of the normal subjects, whereas the bacteria were detected in as high as 14-32 % of the patients with urethritis and cystitis. 6) Among the urethral f l ora, Staphylococcus epidermidis was detected in a great number of the subjects, experimented. Being based on this evidence, investigation were made to determine the inhibiting actions of this microorganism towards the other bacteria. The filter disc method was used in these experiments with the filtrate derived from the cultivation of this species. In a series of the investigations, the following preparatory attempts'were also undertaken satisfactorily with some remarks (1) Influence of culture mediums upon the inhibiting actions ; (2) Relationship between the bacterial count in the culture filtrate and the stage of the inhibition zone formation ; (3) Influences of bacterial count of the experimented bacteria upon the inhibiting actions of the said bacteria ; and so on. 7) The said culture filtrate showed to exert growth inhibiting actions against B. subtilis and streptococcus faecalis significantly, but not against E. coli, Aerobacter aerogenes, Pseudomonas aerogenosa, etc. It was also demonstrated that culture filtrates of M. canklidus and M. varians exert growth inhibiting actions against B. subtilis, respectively. 8) It may be suggested that there is a growth inhibiting substance in a culture filtrate of Staphylococcus epidermidis. Some observations were made to determine the properties of this substance, including the changes of pH of the filtrate, the duration of inhibition zone formation and resistance in heat. From the results obtained, it is probably possible that the said substance is not so stable under the conditions experimented. From the results of all the experiments and investigations, it may be deducted that formation and maintenance of a certain bacterial flora play important roles in respects to the incidences of urethritis and cystitis in female human subjects.|
|Appears in Collections:||Vol.6 No.4|
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