尿路Candidaの高級脂肪酸について

Abstract

Previously Yamamoto et al. reported one case of vesi c a l Candidiasis and the results of a few experiments which have been carried out on Candida in this department. Using 5 Candida strains isolated from vesical Candidiasis and 7 standard Candida strains supplied by the Jynntendo University, their higher fatty acids were analyzed by Fujii's paper choromatographical method in an attempt to find the composition of higher fatty acids in the genus Candida and explore the relationship between the composition of higher fatty acids in the genus Candida and its heat resistance. This communication presents a description of the results thus obtained. Experimental re s ults 1. Higher fatty acids isolated from vesical Candidiasis Five strains that could be identified as C . albicans were used here. Strains No. 1 and 2 showed Rf values of 0.15, 0.49, 0.59, 0.71 and 0.88. A compound of Rf 0.15 was unidentified, while the rest were identified as erucic acid, elaidic acid, oleic acid and linolenic acid. Strain No. 3 had Rf values of 0.15, 0.49, 0.71, 0.83 and 0.90. A compound of Rf 0.15 was unidentified ; compounds of Rf values of 0.49, 0.71 and 0.83 were identified as eluidic acid, oleic acid and linoleic acid ; a compound of Rf 0.90 was unidentified . Strain No. 5 had Rf values of 0.49, 0.71 and 0.88 were identified as erucic acid, oleic acid and linolenic acid. 2. Higher fatty acids in standard Candida strains C. albicans had Rf values of 0.15, 0.39, 0 .49, 0.71 and 0.88. A compound of Rf 0.15 was unidentified ; compounds of Rf values of 0.39, 0.49, 0.71 and 0.88 were identified as stearic acid, erucic acid, oleic acid and linolenic acid . Each of the six strains including C. tropicalis had Rf values of 0.15, 0.49 and 0.88. A compound of Rf 0.15 was unidentified; compounds of Rf values of 0.49 and 0.88 were identified as erucic acid and linolenic acid. Summary and conclusion From analysis o f higher fatty acids in the above Candida strains it can be summarized that as to the standard Candida strains and the Candida strains isolated from visical Candidasis 4 or 5 spots representing higher fatty acids were each demonstrated in C. albicans, while 3 spots were each detected in the other Candida strains . As described in the previous report, C. albicans except C. krusei were more heat-resistnt by one or two degrees than the other Candida strains. Hence it would appear that the composition of higher fatty acids in the genus Candida and its heat resistance are closely related.