腎クリアランスに関する研究 第III篇: 中性子線照射後の腎変化と腎クリアランス

Abstract

(1) As preliminary studies, male mice were irradiated with about 200 rad of either one of neutron or X-ray to the whole body and the radiation damage of the kidney due to relatively small dose of irradiation was observed as well as changes occurred by different radiations. Macroscopic and microscopic findings of the tissue were examined at various intervals from 24 hours through 2 months following the irradiations. No noticeable changes were observed macroscopically, while mild changes in the glomerulus and tubulus and cell infiltration in the interstitials were observed during initial and middle stages, all of which alterations were much less than expected. The death rate was much higher in the group of neutron irradiated mice and the histological changes occurred slightly earlier in this group than that of X-ray irradiated mice. (2) As the next step, male mic e were also irradiated with about 100 rad of neutron so as to observe the changes of macro- and microscopical findings of the kidney as well as to observe the interrelationship of the values of GFR and TmPAH determined by renal clearance. Equally to the results of the preliminary studies, only slight changes, which even disappeared after the middle stages, were observed macro- and microscopically. However, fairly distinct changes were obtained in the results of the renal clearance rates despite slight morphological changes. (3) These results lead us to consider that such small dosage of radiation causes a transient morphological changes in the kidney at the earlier stages followed by rapid regeneration to apparently normal pictures. However it will be supposed that different results might be obtained if the observations can be followed for longer peirods. (4) The renal clearance methods using rats are supposed to be a us e ful mean to determine the changes of renal functions in small animals. However, the methods and the results must be further investigated to achieve a reliability on them.