前立腺亜鉛代謝に関する実験的研究 第2編: 亜鉛試薬の影響について

Abstract

Six different reagents which relate to the zinc metabolism were given to male adult rats. They included Alloxan, Dithizone, Oxine, Nitroso-R Salt (which gives abnormal carbohydrate metabolism through zinc metabolism), BAL, and EDTA-Ca (which gives alteration in zinc metabolism through enzymatic system). Studies were carried out on blood sugar, pancreatic uptake rate of 65Zn, weight and 65Zn uptake rate of the dorso-lateral lobe of the prostatic gland, and histochemical staining of zinc. The results obtained are as follows. 1) A significant correlation between increase of blood sugar and d e crease of pancreatic 65Zn uptake rate was demonstrated , whereas no correlation was found between bl ood sugar and 65Zn uptake rate of the dorso-lateral lobe of the prostatic gland. This means no noticeable effect of systemic abnormality of carbohydrate metabolism (especially Alloxan diabetes) on zinc metabolism of the dorso-lateral lobe of the prostatic gland within 10 days. 2) The 65Zn uptake rate in the dorso-lateral lobe of the prostate w a s supressed with administration of Dithizone, Oxine and N. R. S., of which effect was largest with Dithizone and smallest with N. R. S.. In addition, histological alteration was seen after Dithizone treatment while the other two reagents produced no such changes. Alloxan, EDTA-Ca and BAL showed no effect on uptake rate of 65Zn and histological apperance of the dorso-lateral lobe. This suggests us that activated zinc in the dorso-lateral lobe is not con cerned with the enzymes which impeded by administration of EDTA-Ca or BAL. 3) It was illustrated that Dithizone gives destructive effect t o adenoepithelial cells of the dorso-lateral lobe of the prostatic gland through zinc metabolism. Namely after administration of Dithizone, desquamation of epithelium is seen in 2 days, which became to fill the glandular space in 3 or 4 days. Subsequently necrotic process of the gland and cellular infiltration took place. 4) Histoch e mical staining demonstrated disapperance or decrease of zinc after administration of Alloxan, Dithizone, Oxine and N. R. S.. A diffuse decrease of zinc was seen with Alloxan, Oxine, and N. R. S. while, with Dithizone, initial localized existence of zinc in epithelial cells and glandular space followed by complete disappearance was seen.