エチオニン投与時の白鼠精細管の組織学的並びに組織化学的研究(特に多核性巨細胞の発生起源について)

Abstract

Ethionine, being a biological antagonist of methionine, was given to rats intraperitoneally for a period of 4 weeks and histological and histochemical examinations of the seminal tubuli were performed. The results obtained are summerized as follows. 1) On the basis that daily administration of ethionine produce d marked degeneration of the seminal tubuli, it was found that methionine, one of the indispensable aminoacids, is absolutely necessary for spermatogenesis. 2) Among various sta g es of germinal cells, spermatozoas were found to have the highest sensitivity to ethionine. 3) A mark e d decrease in activity of succinic dehydrogenase was found to precede the degeneration of spermatozoas. 4) Strong ac t ivities of succinic dehydrogenase and adenosine triphosphatase were demonstrated in matured spermatogenic cells. 5) Strong activities of DPN dia p horase and lactic dehydrogenase were found in immature germinal cells. 6) A conclusion was drawn that multinucleated giant cell is derived from either spermatogonia or spermatocyte, since the cell has weak activity of succinic dehydrogenase but has strong activities of DPN diaphorase and lactic dehydrogenase as well as positive glycogen reaction.