ラット前立腺の組織化学 1: 下垂体,副腎,睾丸系の影響

Abstract

Using Wistar-strain male rats, weight and histochemical changes of the prostatic gland, testicles, seminal vesicle and adrenal glands were examined after extirpation of each endocrine organ (pituitay, adrenals and testicles) and administistion of various hormones. It was found that stainability is well coincided with the sexual functions. 1) The lateral portion of the dorsolateral lobe of the prostati c gland well represented sexual functions and showed the best stainability. The histochemical activities of ACP, RNA, Zn, and SD run parallel with prostatic functions but activity of ALP showed a reverse relationship. 2) Stainability of ALP, ACP and RNA in seminal tubulus of the testicle represented its spermatogenetic function. 3) In seminal v esicle, to which SD stain was only attempted, strong activity was demonstrated in epithelium of which stainability yielded to be concured with that of prostatic gland. 4) In adrenal cortex stainabilities of Sudan III and 17 KS generally coincided with its functions. 5) With the days elapsed after hypophysectomy, weight and histochemical activities of the testicles and adnexal organs were decreased, especially in the prostatic gland which showed marked hypofunction after 4th weeks from the operation. Administration of Tp following hypophysectomy resulted a marked increase in weight of the adnexal organs and slight recovery of testicular spermatogenic function. Administration of Eb following hypophysectomy did not show decrease in weight of the testicle and adnexal organs but decrease in histochemical activities. 6) With administration of anterior pituitary hormones to normal rats, effects on testicles and adnexal organs were recognized in the following order of PMS, HCG, hypophorin, prolactin and controls. Adrenal functions were accerelated in every instances. 7) With adrenalectomy, the testicles and adnexal organs showed decrease s in both their weight and function, which were failed to be corrected by administration of cortisone. With administration of large doses of cortisone, these suppressive effects occurred more rapidly and predominantly. ACTH administration resulted marked increases in weight of the testicles and adnexal organs with a little accerelation of their functions. Combination therapy with cortisone and sexual hormones gave more pronounced effects of the sexual hormones on the testicles and adnexal organs, of which effects yielded to be suppressed by cortisone. 8) With ca s tration, decreases in both of weight and function of the prostatic gland and seminal vesicles were demonstrated, while no significant change was seen in the adrenal glands. With large doses of Tp after castration, unexpectedly slight increase was only observed in weight of the prostatic gland. With Eb, no much change was also demonstrated in its weight and function. 9) With administration of large dose of Tp, 0.5 mg per day, to normal rats, weight and functions showed increases in the prostatic gland, decreases in the testicles and slight decrease in the adrenal glands. With administration of Eb, 0.02 mg per day, to normal rats, weight and functions showed decreases in the testicles and adnexal organs and increase in the adrenal glands.