前立腺のDNA代謝 第1編: 正常ラット前立腺のDNA代謝

Abstract

Using male rats of Wistar strain, synthesis of DNA and cell division, mode of adenoepithelial cells and cells of interstitial tissue and prostatic base in the ventral lobes of the prostatic gland were studied by means of autoradiography using 3H-Thymidine. 1) With injection of 3H-Thymidine into the ventral lobe of the ra t prostatic gland, labelling of nuclei in the interstitial tissue begun to be observed in 15 minutes. 2) Labelling of epithelial cells and cells of the base begun to b e recognized in 30 minutes. 3) Incorporation into epithelial cells showed irregular distribution without any specific trend of pattern nor specific localization. No distinct relationship was also found between morphological apperance of epithelial nuclei and labelling. 4) The duration between DNA synthesis an d mitosis in epithelial cells of ventral lobes in rats was supposed to be 6 hours, since labeled mitotic cells became to be found in 6 hours after injection in the epithelial cells. 5) Based on the result of 1 hour 3H-in d ex as 0.6, to of epithelial cells was estimated to be about 42 days. 6) Labelling of epithelial nuclei after 6 hours of injection frequfently showed coupled arrangement. Occasionally, incorporation was demonstrated in nuclei of cells which appeared to be isolated above the cell layer. These ones seemed to represent cells, of which secreting function is not developed yet. 7) In the cells of inter s t itial tissue, number of labeled cells did not correlate to time lapsed, probably due to presence of many different kind of cells and/or migrated cells from the surrounding tissues. 8) In the cells of p r ostatic base, proliferation of the cells and mean grain counts were not correlated. 9 ) There seems to be no reutilization of labeled 3E1 b y prostatic epithelial cells.