Dithizoneの前立腺に対する影響 第1編: Dithizoneのラット睾丸,精嚢腺,前立腺に対する影響について

Abstract

So far many reports indicated that the prostatic gland contains fairly large amount of zinc. This report deals with the results of observations on changes of the prostatic gland, testicle and seminal vesicle of rats after administration of Dithizone, one of the zinc-chelating agents, at the dose of 200 mg/kg body weight. Change of DNA synthesis in the acinus wall of the prostatic gland after Dithizone treatment by means of radioautogram using 3H-thymidine was also studied. The results are summarized as follows : 1) No noticeable change was found on weight of the rats' testicle, seminal v e sicle and prostatic gland after administration of Dithizone. 2) Dithizone-treated rats showed no re m arkable histological change in the prostatic gland except for its median lobe. 3) Among the median l o be, exfoliation and destruction of glandular epitheliar cells was markedly observed in the lateral and dorsolateral lobes, but less observed in the dorsal lobe. 4) T he changes in acinus wall of the lateral and dorsolateral lobes became recognized in about 12 hours after Dithizone administration, became exforliated and destructed in 3 to 5 days and showed regeneration in 7 to 14 days. 5) In the testicle of the Dithizone-treated r ats, changes of interstitial tissue, damages of spermatozoid and appearance of giant cells were observed. However no noticeable change was found in the seminal vesicle. 6) 3H-thymidine incorp o ration was not differ among the anterior, median and posterior lobes. The grain counts of the incorporated cells were mostly between 4 to 20. 7) After Dithizone administration, however, incorporation of 3H-thymi d ine and its grain counts decreased during the stage when acinus wall showed exfoliation and destruction histologically. Abrupt increase in 3H-thymidine incorporation and slight increase in its grain counts were demonstrated during the stage of regeneration in 7 to 14 days after administration. 8) Based on the results of 3H-thymidine incorporation, it was supposed that the mechanisms of Dithizone on the prostatic epithelial cells are attributed to its inhibitory effect on nucleic acid synthesis, abnormal nucleic acid composition, abnormal nucleic acid metabolism and relative decrease of DNA synthetic neriod through zinr