3H-Thymidineによる早期膀胱癌の臨床病理学的研究

Abstract

Bladder carcinoma arising from a transitional epith e lium is the particular kind of carcinoma, which in early stage is proliferative with papillary form and then is apt to develope multiple and malignant characters with chronic reparative proliferation. Therefore, for the planning of the treatment o f bladder carcinoma in its early stage it is important to examine the potentially malignant distribution and degree of the papillary-formed cells. In order to distinguish the malignitude of hyperplasia from carcinoma in situ, observations were made on the clinicopathological change of the constituent cell of the bladder carcinoma in early stage and papilloma not only with hematoxylin-eosin stained specimen but also by means of 3H-thymidine radioautography following in vivo and in vitro labelings determining the distribution of labeled cells, labeling index and grain count. The results obtained are summarized as follows . 1) With papillary proliferation, 3H-thym i dine labeled cells were more frequently found in the intercellular layer than in the basal cell layer. 2) Labeling index was found to be 1-2% in papilloma, 0.6-3.1% in carcinoma of early stage and 10-20% in multiple cases. The labeled cells in such situations distributed in the upper area of the intercellular layer. It was suggested that there may exist a generation zone in the upper layer rather than the basal layer. 3) 3H-thymidine radioautography of b ladder carcinoma with in vivo labeling brought a better chinical results than with in vitro method.