簡便な尿中Glycosaminoglycans(GAG)測定法(Dimethylmethylene-blue(DMB)変法)について

Abstract

尿中GAG濃度の測定を簡易化し, 安全で短時間に測定でき, かつ容易に日常臨床検査に利用できるようにmodified DMB法を開発した。この測定法における測定値の再現性および回収率は満足できるものと思われ, 今後スクリーニング法として十分利用しうると思われた

The measurement of glycosaminoglycans (GAG) in urine is very important because GAG is an essential constituent of urinary diseases. Since previously reported methods of determination of GAG are quite complicated, dangerous or not stable, we developed a very simple and rapid method to estimate the content of GAG in urine using a microplate and microplate reader. An aliquot of diluted urine is mixed with a 1, 9, dimethylmethylene blue (DMB) dye. The assay is based on the metachromatic shift in absorption maximum which occurs when sulfated glycosaminoglycans are added to the dye. The recovery of GAG is good. Other urinary compounds such as sugar, protein and blood did not influence the measurement of GAG. A good correlation was obtained between the modified DMB method and Blumenkrantz and Asboe-Hansen's method (r = 0.737, p < 0.001). The accuracy of this method makes it useful for screening urinary diseases.