Growth of human tumor xenografts on chorioallantoic membrane of chick embryo

Abstract

腎細胞癌の78.6%, 尿路上皮癌の77.8%, 精巣腫瘍の66.7%, 結腸腺癌, 及び卵巣腺癌は移植に成功したが, 前立腺癌は全て失敗した。組織学的に膜上で生存している細胞は, 構造が良く保たれている類器官を形成していた。しかし, 癌巣の生存力は壊死組織周囲の炎症性変化の影響を受けなかった。腎細胞癌は生存し, 瀰漫性に顕著に血管を形成していた。膜上の癌細胞の%BrdU(ブロモデオキシウリジン)標識指数と元の腫瘍の%Ki-67指数との間には強い相関が認められた。癌巣の大小によっては%BrdU指数に差異がなかった。接種後8日間成長状態は一定に留まっていた。生存している癌巣の成長能力はインキュベーション中不変

We evaluated the success rates of transplantation and growth conditions of 33 human tumor xenografts transplanted on the chorioallantoic membrane of chick embryos. Eleven out of 14 renal cell carcinomas (78.6%), 7 out of 9 urothelial carcinomas (77.8%), 2 out of 3 testicular tumors (66.7%), an adenocarcinoma of the colon and an adenocarcinoma of the ovary but none of the 5 prostatic carcinomas were transplanted successfully. Histologically, carcinoma cells survived on the chorioallantoic membrane forming organoids the structure of which was well preserved. Several regions of the grafts frequently became necrotized. However, the viability of carcinoma nests was not influenced by the inflammatory changes surrounding necrotic tissues. Renal cell carcinomas survived diffusely with prominent angiogenesis. Immunostaining with anti-bromodeoxyuridine (BrdU) and Ki-67 monoclonal antibodies demonstrated a strong correlation between the %BrdU labeling index of the cancer cells on the chorioallantoic membrane and %Ki-67 index of the original tumors. No difference in %BrdU indexes was found between small and large cancer nests. Growth conditions remained constant for 8 days after inoculation. The growth potential of cancer nests surviving on the chorioallantoic membrane, which was identical to that of the original carcinomas, appeared to be unchanged during incubation. However, it might be difficult to exploit the chorioallantoic membrane for anticancer chemosensitivity tests except for renal cell carcinoma since few cancer cell nests were produced in spite of the high transplantation success rates.