膀胱移行上皮癌におけるPCNA/cyclin, p53, C-erbB-2の発現と組織学的悪性度に関する検討

Abstract

通常のホルマリン固定パラフィン包埋標本を用いた検討でも各抗体の陽性率はgradeやstageおよび生存率とも相関を認めている。しかし, 生存率についてstage群別に分けて検討したところ, 染色性による差は認められなかった

It is important to know the proliferating ability and the malignant potential of tumor tissues. We have examined the expression of PCNA/cyclin, p53 and C-erbB-2 in transitional cell carcinoma of the human urinary bladder by an immunohistochemical method, and compared the results with the histological grade, stage and survival rate. Immunohistochemical studies, using monoclonal and polyclonal antibodies, on these proteins were performed with formaline fixed-paraffin sections of tumor tissue from 40 patients with bladder cancer. Generally, a higher grade and higher stage tumors expressed PCNA/cyclin, p53 and C-erbB-2 with a greater frequency than the tumors with a lower grade and lower stage and strongly stained cases had a lower survival rate than weakly stained cases. These findings suggest that the detection of each antigen is useful for estimating the malignant potential of transitional cell carcinoma as the adjuvant studies, because of its applicability to paraffin-embedded tissue sections and its simple, rapid technique.