腎細胞癌及び膀胱腫瘍のマイクロサテライト領域における遺伝子変異の検討

Abstract

2番染色体短腕(D2S123), 3番染色体短腕(D3S1067), 17番染色体短腕(TP53)の3種のプライマーを使用して, 膀胱腫瘍及び腎細胞癌について染色体不安定性を検討した.染色体不安定性試験は, 腎細胞癌で17例中5例, 膀胱腫瘍では14例中3例に陽性であった

We investigated the reduction in the accuracy of DNA replication and repair (i.e. genetic instability) in urinary tract malignancy using microsatellite regions. The subjects were 17 patients with renal cell carcinoma and 14 with bladder tumors. After polymerase chain reaction (PCR) was performed with FITC-labeled primers, CA repeats were analyzed. The primers used were D2S123, D3S1067, and TP53. Tissue positive for all 3 primers was considered to show a replication error (RER). Genetic instability was found in 5 out of 17 patients with renal cell carcinoma (29%) and 3 out of 14 patients with bladder tumors (21%). Among the bladder tumor patients, 2 were positive for only TP53 and 1 was considered to have RER. Among the patients with renal cell carcinoma, one was positive only for D3S1067 and the other 4 were considered to have RER.