尿中より分離された各種細菌による実験的膀胱結石

Abstract

ウィスター系の雄ラットの膀胱に手術的に, 亜鉛リング及び各種細菌を接種し, 感染結石形成を調べた. 1)P.mirabilis接種ラットで尿pHの上昇が著しく, S.saprophyticusを接種したラットでも軽度の尿pHの上昇がみられた.E.coli, P.aeruginosa及びK.pneumoniaeを接種したラットでは尿pHの変化はなかった. 2)P.mirabilis及びS.saprophyticus接種ラットでは, それぞれ100%, 60%で膀胱結石がみられ, 成分はリン酸マグネシウムアンモニウムであった.P.aeruginosa, K.pneumoniae接種ラットでは, ごく僅かな結石がみられたのみで, E.coliでは, 結石はみられなかった

To investigate the formation of infection stones by 5 bacterial species isolated from patients with urinary calculi, such as Proteus mirabilis, Staphylococcus saprophyticus, Pseudomonas aeruginosa, Klebsiella pneumoniae and Escherichia coli, a zinc disc was implanted and 10(6) bacteria were surgically inoculated at the same time into the bladder of male Wistar rats (10 rats per group). Urinary pH was measured on days 1, 3, 5 and 7. The rats were killed on day 7 and the bladder examined for the formation of infection stones. Infection stones were formed in all rats inoculated with P. mirabilis (mean stone weight 29.7 mg), and in 60% of those inoculated with S. saprophyticus (9.7 mg). Small bladder stones were formed in 30% of the rats inoculated with K. pneumoniae and 25% of those inoculated with P. aeruginosa. No bladder stones were formed in the rats inoculated with E. coli. Urinary pH was increased by P. mirabilis and S. saprophyticus but was unaffected by the other species. In conclusion, P. mirabilis and S. saprophyticus play a significant role in the formation of infection stones.