腎細胞癌におけるPCNA標識率による増殖能とIL-6分泌の検討

Abstract

腎細胞癌におけるPCNA標識率, grade, stageと術前血清中IL-6値及び抗IL-6抗体を用いた腎細胞癌組織のIL-6分泌動態との関連について検討した. 1)PCNA標識率はgradeの上昇に伴い有意(P<0.05)に高値を示した.low stage群とhigh stage群においては有意差は認めなかった. 2)免疫組織学的検討より, IL-6の分泌陽性細胞のコロニー数は各grade間及び2つのstage群間において有意差は認めなかった. 3)血清IL-6値は各grade間及び2つのstage群間において有意差は認めなかった. 4)相関係数による検討にては, PCNA標識率と陽性コロニー数, 血清IL-6値とCRP及びESRの間に相関が認められた.以上から, 腎細胞癌組織内にてIL-6は分泌され腫瘍の増殖, 進展に関与する腫瘍側因子と推測される.血清IL-6値は腎細胞癌をはじめとし, 担癌宿主の免疫担当細胞等の因子も関与した総合的な分泌の結果と考える

The relationship between the proliferating cell nuclear antigen (PCNA) expression in renal cell carcinoma (RCC) determined by immunohistochemical staining using the PC10 clone, and the preoperative serum interleukin-6 (IL-6) value determined by ELISA was examined. The secretion of IL-6 in RCC was also examined immunohistochemically using an anti-IL-6 antibody. The PCNA labeling rate was significantly higher in grade 3 tumors than in grade 1 tumors (p < 0.05), but there were no significant differences between the other grades or TNM stages. No significant correlation was obtained between the serum level of IL-6 or the positive cell rate of IL-6 and the pathological grade of the RCC. A correlation was observed between the PCNA labeling rate and positive cell rate, and between the serum IL-6 value and CRP or ESR. In conclusion, the secretion of IL-6 was detected in RCC tissue, and was suggested to be a tumor factor responsible for the growth and spread of RCC. The serum IL-6 value is considered to reflect the total secretion of IL-6 produced by the RCC and accessory cells, i.e., monocyte-macrophage lineage cells, endothelial cells and fibroblasts.