ラット前立腺におけるコレステロール蓄積に関する検討

Abstract

1)ラットの前立腺腹葉開口部の尿道に凍結処置を加え, また高cholesterol食で飼育し, 前立腺肥大症の実験モデルの作成を試みた.2)ラット前立腺腹葉における高cholesterol食単独の影響は認められなかった.3)凍結処置によって前立腺重量はコントロールに比べ減少し, 肥大を作成しえなかった.凍結処置後に高cholesterol食で飼育した群では単位組織重量当たりのcholesterol含有量が増加した.3)前立腺に適切な分泌障害を作成し, 高cholesterol食で飼育すれば前立腺におけるcholesterolの蓄積が得られるものと考えられた

An experimental model of prostatic hypertrophy was created by freezing the urethra at the opening of the ventral prostate in rats and administering a cholesterol-rich diet to induce cholesterol accumulation. Four-week-old Fisher rats were divided into a control group, a cholesterol-rich diet group (by adding 1% cholesterol to the diet), a frozen group, and a frozen and cholesterol-rich diet group, and were reared for 5 months. The animals were killed, and the ventral prostate was removed, weighed, and the lipid was extracted. Cholesterol content was measured by an enzymatic method. A cholesterol-rich diet without freezing had no effects on the ventral prostate, and after freezing alone, the weight of the prostate was smaller than that of the control group. However, in the group cholesterol-rich diet after urethral freezing, the cholesterol content per mg of tissue increased. This indicates that cholesterol accumulation can be induced in the prostate by administering a cholesterol-rich diet after creating the proper secretory disturbance.