新しい酵素法によるポリアミン測定について 第7報: 本法における組織中ポリアミン分別定量の基礎的検討

Abstract

1)第1報において報告した新しい簡便な酵素法による尿中ポリアミン分別定量法を変法し, 腎皮質組織中ポリアミンの分別定量を行った.2)添加回収試験の結果は, diamine 104.5±7.4%, spermidine 104.7±23.3%, spermine 104.8±7.5%であり, いずれも良好な成績を示した.3) HPLC法との比較試験では, diamine, spermidine, spermineのいずれも良好な相関関係が得られた

The enzymatic method for isolation and determination of urinary polyamines was modified to measure the polyamines in tissue. High recovery rates of polyamines in tissue by the enzymatic method were obtained, namely, 104.5 +/- 7.4% for diamine, 104.7 +/- 23.3% for spermidine and 104.8 +/- 7.5% for spermine. The results obtained by this method correlated well with those obtained by high performance liquid chromatography, and a close correlation was demonstrated for all fractions: diamine r = 0.8991, y = 0.9916x + 0.0499; spermidine r = 0.8936, y = 1.2073x + 0.1005; spermine r = 0.8921, y = 1.2200x-0.2194, (n = 13).