ヌードマウス可移植性ヒト腎細胞癌株に対するα-インターフェロンと抗癌剤との併用療法の効果

Abstract

ヌードマウスを用いてIFNと各種抗癌剤との併用療法の効果を検討した.1)移植腫瘍重量は無投与群の経時的増殖に比べ, IFN単独投与群では増殖抑制が, また抗癌剤併用群ではさらに強い抑制がみられた.2) Battele-Columbus方式に準じた判定では, ADM, PEPは有効, 5-FUは無効であり, ADM, PEPはIFNとの協調効果があった.3)組織学的にはIFNの細胞増殖抑制効果が推測された

The direct antitumor effects of combined administration of alpha-interferon and chemotherapeutic agents against the human tumor cell line derived from renal cell carcinoma were examined in vivo as xenograft in nude mice. The administration regimen was as follows: Human lymphoblastoid interferon (HLBI) was injected intramuscularly (1 x 10(5) IU/mouse/day) every day for 14 days. Peplomycin (PEP), adriamycin (ADM), or 5-fluorouracil (5-FU) was also administered at a dose of one third of their LD50. We evaluated the effect 20 days after initial administration using the ratio of mean tumor weight. Combined administration of HLBI and PEP or ADM was determined effective and inhibited tumor growth more strongly than HLBI alone or control. Furthermore, the histopathological examination suggested that the effect of combined administration was cytostatic rather than cytolytic.