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Title: 膀胱腫瘍間質におけるプロテオグリカンの免疫組織化学的局在
Other Titles: Immunohistochemical localization of proteoglycans in interstitial elements of human bladder cancer
Authors: 高村, 真一  KAKEN_name
三宅, 弘治  KAKEN_name
竹内, 純  KAKEN_name
Author's alias: Takamura, Shinichi
Miyake, Kouji
Takeuchi, Jun
Keywords: Immunohistochemistry
Large proteoglycans
Small proteoglycans
Chondroitin 6-sulfate
Bladder tumor
Issue Date: Apr-1991
Publisher: 泌尿器科紀要刊行会
Journal title: 泌尿器科紀要
Volume: 37
Issue: 4
Start page: 329
End page: 334
Abstract: プロテオグリカンのコア蛋白および糖側鎖を認識する6種類のモノクローナル抗体を用いて免疫組織化学的検索を行った.1)非腫瘍部膀胱組織では粘膜上皮細胞表面にヘパラン硫酸が認められ,粘膜下層では主として側鎖にコンドロイチン4硫酸を有するsmall PGがみられたが,デルマタン硫酸,コンドロイチン6硫酸およびlarge PGはほとんどみられなかった.2)膀胱腫瘍組織では腫瘍細胞表面にヘパラン硫酸がみられず,腫瘍固有間質には側鎖にコンドロイチン4硫酸またはコンドロイチン6硫酸を有するlarge PGが主としてみられ,デルマタン硫酸およびsmall PGに対する反応はほとんどなかった.3)コンドロイチン6硫酸の存在を示す抗体3B3の染色で,非浸潤性腫瘍の一部に腫瘍基底部に接する結合組織のみならず粘膜下結合組織,筋層部結合組織にまで広範に陽性所見を示すものが存在した.このような非浸潤性腫瘍は,将来浸潤性腫瘍に進展する可能性がある
The immunohistochemical localization of glycosaminoglycan side chains and core protein of proteoglycan was observed, using monoclonal antibodies, on 8 specimens of non-tumor bladder tissues and 26 specimens of bladder tumors obtained from total cystectomy or transurethral resection of bladder tumor (TUR-Bt). In non-tumor tissues of human bladder examined, the surface of urothelial epithelium consisted of heparan sulfate as revealed with antibody HepSS-1, and submucosal interstitial elements consisted mainly of small proteoglycan having chondroitin 4-sulfate side chains as revealed with antibodies 6B6 and 9A2, respectively. On the other hand, in bladder tumors examined, the interstitial fibrous elements, the so-called "specific stroma" in cancer cell nests, consisted mainly of large proteoglycans having chondroitin 4-sulfate or 6-sulfate side chains as revealed with antibodies 2B1, 9A2 and 3B3, respectively. Antibodies 2B1 and 3B3 are considered to be useful to demonstrate the involvement by invasive growth of bladder tumor cells.
PubMed ID: 1891991
Appears in Collections:Vol.37 No.4

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