皮膚吸引浸出液の生化学検査試料としての有用性について

Abstract

皮膚を抜気吸引して得られる微量の浸出液に注目し, その生化学検査試料としての有用性を検討するために実験を行った.対象は雑種雌成犬とし, 検査項目は血漿クレアチニン, BUNに特に注目した.1)吸引に適した回路内圧は300 mmHgであり, 皮膚の同一部位で行った6時間の吸引実験では, 継続して安定した液量が得られた.2)尿毒症モデルでは, 臨床上問題となる全濃度域で, 浸出液のクレアチニン, BUN濃度は血漿濃度とよく一致した.3)吸引実験後の皮膚は1週間後に良好な角質の再生を示し, 4週間後には完全に元の状態に回復していた.4)吸引浸出液の血漿クレアチニン, BUN濃度測定試料としての信頼性は高く, とくにバイオセンサ試料として適していると考えられた

We examined whether the suction effusion fluid could be used as a new sample for blood biochemistry. The fluid was obtained from the skin surface of female mongrel dogs by transcutaneous suction after removal of the horny substance. The appropriate intraluminal pressure for suction was 300 mmHg at which the effusion fluid flow rate was 0.6 microliter/min/cm2. The blood biochemistry examination revealed that the creatinine (Cr) and urea (BUN) concentrations in the suction effusion fluid were similar to those in plasma and that the protein and lipid concentrations were much smaller than those in plasma. The six-hour study showed that the effusion fluid could be obtained for hours at the same skin area and that the Cr and BUN concentrations were consistently close to those in plasma. In uremia models, the Cr and BUN concentration in the suction effusion fluid was similar to and had an extremely high correlation (r = 0.985 and 0.982 respectively) to that in plasma through out the range for clinical practice. The skin biopsy revealed that the regeneration of the horny substance had started at one week after the suction and complete recovery was seen at four weeks. The invasiveness of the suction seemed to be small. The suction effusion fluid is a reliable material for measurement of Cr and BUN in plasma and, as it contains much less proteins and lipids, will be a good sample for biosensors.